+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Assembly and immunogenicity of coronavirus-like particles carrying infectious bronchitis virus M and S proteins



Assembly and immunogenicity of coronavirus-like particles carrying infectious bronchitis virus M and S proteins



Vaccine 31(47): 5524-5530



Infectious bronchitis virus (IBV) as an avian coronavirus is still posing a persistent and imminent threat to the poultry industry worldwide. Here we report that transfection of Sf9 cells with a single recombinant baculovirus encoding M and S proteins resulted in the assembly of IBV VLPs; this is the first report that S protein plus M protein alone were able to be assembled into VLPs for coronaviruses. We further showed that the generated IBV VLPs could induce humoral immune responses in a level comparable to that of inactivated IBV vaccine, and more importantly the IBV VLPs could elicit significantly higher cellular immune responses than the inactivated IBV vaccine. In summary, the assembly of IBV VLPs with M and S proteins provided a simple strategy for generating VLPs for coronaviruses, and the generated IBV VLPs laid a feasible foundation for the development of an effective vaccine against infection of IBV in the future.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 051649025

Download citation: RISBibTeXText

PMID: 24099870

DOI: 10.1016/j.vaccine.2013.09.024


Related references

Assembly and immunogenicity of baculovirus-derived infectious bronchitis virus-like particles carrying membrane, envelope and the recombinant spike proteins. Biotechnology Letters 38(2): 299-304, 2016

The missing link in coronavirus assembly. Retention of the avian coronavirus infectious bronchitis virus envelope protein in the pre-Golgi compartments and physical interaction between the envelope and membrane proteins. Journal of Biological Chemistry 276(20): 17515-17523, 2001

Coronavirus proteins: Biogenesis of avian infectious bronchitis virus virion proteins. Structure and function of the oligosaccharides of the avian infectious bronchitis virus glycoproteins. Journal of Virology 44(3): 794-812, 1982

Proteomic analysis of purified coronavirus infectious bronchitis virus particles. Proteome Science 8: 29, 2010

Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus. Journal of Veterinary Science 15(2): 209-216, 2014

Coronavirus proteins: biogenesis of avian infectious bronchitis virus virion proteins. Journal of Virology 44(3): 794-803, 1982

Interaction of the coronavirus infectious bronchitis virus membrane protein with beta-actin and its implication in virion assembly and budding. Plos one 4(3): E4908, 2009

Changes in nucleolar morphology and proteins during infection with the coronavirus infectious bronchitis virus. Cellular Microbiology 8(7): 1147-1157, 2006

Structural analysis of virion proteins of the avian coronavirus infectious bronchitis virus. Journal of Virology 42(1): 208-219, 1982

Sialic acid binding properties of soluble coronavirus spike (S1) proteins: differences between infectious bronchitis virus and transmissible gastroenteritis virus. Viruses 5(8): 1924-1933, 2013

Coronavirus proteins: structure and function of the oligosaccharides of the avian infectious bronchitis virus glycoproteins. Journal of Virology 44(3): 804-812, 1982

Neither the RNA nor the proteins of open reading frames 3a and 3b of the coronavirus infectious bronchitis virus are essential for replication. Journal of Virology 80(1): 296-305, 2006

Physical interaction between the membrane (M) and envelope (E) proteins of the coronavirus avian infectious bronchitis virus (IBV). Advances in Experimental Medicine and Biology 494: 595-602, 2001

Coronavirus infectious bronchitis virus partial amino terminal sequencing of spike polypeptide s 2 identifies the sequence arginylarginylphenylalanylarginylarginine at the cleavage site of the spike precursor propolypeptide of infectious bronchitis virus strains beaudette and m 41. Virus Research 4(2): 133-144, 1986

Coronavirus multiplication: locations of genes for virion proteins on the avian infectious bronchitis virus genome. Journal of Virology 50(1): 22-29, 1984