Association of Rice gall dwarf virus with microtubules is necessary for viral release from cultured insect vector cells
Wei, T.; Uehara-Ichiki, T.; Miyazaki, N.; Hibino, H.; Iwasaki, K.; Omura, T.
Journal of virology 83(20): 10830-10835
2009
ISSN/ISBN: 0022-538X PMID: 19640979 DOI: 10.1128/jvi.01067-09
Accession: 051680856
Vector insect cells infected with Rice gall dwarf virus, a member of the family Reoviridae, contained the virus-associated microtubules adjacent to the viroplasms, as revealed by transmission electron, electron tomographic, and confocal microscopy. The viroplasms, putative sites of viral replication, contained the nonstructural viral proteins Pns7 and Pns12, as well as core protein P5, of the virus. Microtubule-depolymerizing drugs suppressed the association of viral particles with microtubules and prevented the release of viruses from cells without significantly affecting viral multiplication. Thus, microtubules appear to mediate viral transport within and release of viruses from infected vector cells.