+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Automated serum peptide profiling using novel magnetic C18 beads off-line coupled to MALDI-TOF-MS



Automated serum peptide profiling using novel magnetic C18 beads off-line coupled to MALDI-TOF-MS



Proteomics. Clinical Applications 1(6): 598-604



Serum peptide profiling by MS is an emerging approach for disease diagnosis and biomarker discovery. A magnetic bead-based method for off-line serum peptide capture coupled to MALDI-TOF-MS has been recently introduced. However, the reagents are not available to the general scientific community. Here, we developed a protocol for serum peptide capture using novel magnetic C18 beads, and automated the procedure on a high-throughput magnetic particle processor. We investigated bead equilibration, peptide binding and peptide elution conditions. The method is evaluated in terms of peaks counts and reproducibility of ion intensities in control serum. Overall, the DynaBead-RPC18-based serum sample processing protocol reported here is reproducible, robust and allows for the detection of similar to 200 peptides at m/z 800-4000 of serum that was allowed to clot for 1 h. The average intra-experiment %CV of normalized ion intensities for crude serum and 0.5% TFA/0.15% n-octyl glucoside-treated serum, respectively, were 12% (range 2-38%) and 10% (3-21%) and the inter-experiment %CVs were 24% (10-53%) and 31% (10-59%). Importantly, this method can be used for serum peptide profiling by anyone in possession of a MALDI-TOF instrument. In conjunction with the KingFisher (R) 96, the whole serum peptide capture procedure is high-throughput (similar to 20 min per isolation of 96 samples in parallel), thereby facilitating large-scale disease profiling studies.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 051726920

Download citation: RISBibTeXText

PMID: 21136711

DOI: 10.1002/prca.200600483


Related references

MALDI-TOF serum profiling using semiautomated serum peptide capture with magnetic reversed phase (C18) beads. Methods in Molecular Biology 790: 3, 2011

Detection of Leishmania donovani infection using magnetic beads-based serum peptide profiling by MALDI-TOF MS in mice model. Parasitology Research 110(3): 1287-1290, 2012

Serum peptide profiling using MALDI mass spectrometry: avoiding the pitfalls of coated magnetic beads using well-established ZipTip technology. Proteomics 7(Suppl. 1): 77-89, 2007

Serum peptide profiling by magnetic particle-assisted, automated sample processing and MALDI-TOF mass spectrometry. Analytical Chemistry 76(6): 1560-1570, 2004

Proteomic Profiling of Invasive Ductal Carcinoma (IDC) using Magnetic Beads-based Serum Fractionation and MALDI-TOF MS. Journal of Clinical Laboratory Analysis 29(4): 321-327, 2015

A novel and rapid approach to protein expression profiling of cerebrospinal fluid from medulloblastoma patients using functionalized magnetic beads, AnchorChipTM technology, MALDI - TOf and MALDI - TOF/TOF mass spectrometry. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 751 3, 2003

Detection of murine toxoplasmosis using magnetic bead-based serum peptide profiling by MALDI-TOF MS. Vector Borne and Zoonotic Diseases 12(6): 462-466, 2012

A new approach to profile ovarian cancer serum using functionalized magnetic beads, AnchorChipTM technology, MALDI-TOF MS, MALDI-TOF/TOF MS and bioinformatics. Molecular & Cellular Proteomics 2(7 Suppl.): S31, 2003

Impact of experimental and demographic variables in serum peptide profiling based on magnetic bead and MALDI-TOF mass spectrometry. Clinica Chimica Acta; International Journal of Clinical Chemistry 412(1-2): 112-119, 2011

Early diagnosis of Irkut virus infection using magnetic bead-based serum peptide profiling by MALDI-TOF MS in a mouse model. International Journal of Molecular Sciences 15(4): 5193-5198, 2014

Quality control based on isotopic distributions for high-throughput MALDI-TOF and MALDI-FTICR serum peptide profiling. Journal of the American Society for Mass Spectrometry 21(9): 1515-1525, 2010

Identification of pathogenic bacteria in human blood using IgG-modified Fe 3 O 4 magnetic beads as a sorbent and MALDI-TOF MS for profiling. Mikrochimica Acta 185(12): 542, 2018

Proteomic expression profiling and identification of serum proteins using immobilized trypsin beads with MALDI-TOF/TOF. Journal of Proteome Research 8(9): 4182-4192, 2009

Photocleavable peptide-conjugated magnetic beads for protein kinase assays by MALDI-TOF MS. Bioconjugate Chemistry 21(10): 1917-1924, 2010

Enhanced neuropeptide profiling via capillary electrophoresis off-line coupled with MALDI FTMS. Analytical Chemistry 80(16): 6168-6177, 2008