+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Breaking the diffraction barrier: super-resolution imaging of cells



Breaking the diffraction barrier: super-resolution imaging of cells



Cell 143(7): 1047-1058



Anyone who has used a light microscope has wished that its resolution could be a little better. Now, after centuries of gradual improvements, fluorescence microscopy has made a quantum leap in its resolving power due, in large part, to advancements over the past several years in a new area of research called super-resolution fluorescence microscopy. In this Primer, we explain the principles of various super-resolution approaches, such as STED, (S)SIM, and STORM/(F)PALM. Then, we describe recent applications of super-resolution microscopy in cells, which demonstrate how these approaches are beginning to provide new insights into cell biology, microbiology, and neurobiology.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 051860979

Download citation: RISBibTeXText

PMID: 21168201

DOI: 10.1016/j.cell.2010.12.002


Related references

Breaking the barrier: fluorescence microscopy with diffraction-unlimited resolution. Chromosome Research 15(Suppl. 2): 55, 2007

Real-time up-converter from HDTV to 4K with super-resolution: Breaking the Nyquist frequency barrier. Journal of the Society for Information Display 21(2): 89-93, 2013

Chapter 12: Nanoscale biological fluorescence imaging: breaking the diffraction barrier. Methods in Cell Biology 89: 329-358, 2009

Diffraction-limited imaging and beyond; the concept of super resolution. Geophysical Prospecting 59.3, 2011

Conical diffraction illumination opens the way for low phototoxicity super-resolution imaging. Cell Adhesion & Migration 8(5): 430-439, 2015

Photoactivatable fluorescent proteins for diffraction-limited and super-resolution imaging. Trends in Cell Biology 19(11): 555-565, 2009

Imaging the behavior of molecules in biological systems: breaking the 3D speed barrier with 3D multi-resolution microscopy. Faraday Discussions 184: 359-379, 2016

Breaking the acoustic diffraction limit via nonlinear effect and thermal confinement for potential deep-tissue high-resolution imaging. Applied Physics Letters 102(6): 63703-63703, 2013

Confocal super-resolution imaging of the glomerular filtration barrier enabled by tissue expansion. Kidney International, 2017

Super-resolution imaging of subcortical white matter using stochastic optical reconstruction microscopy (STORM) and super-resolution optical fluctuation imaging (SOFI). Neuropathology and Applied Neurobiology, 2017

Towards breaking the spatial resolution barriers: An optical flow and super-resolution approach for sea ice motion estimation. Isprs Journal of Photogrammetry and Remote Sensing 138: 164-175, 2018

High-resolution X-ray diffraction study of the chromite (Mg (sub 0.60) Fe (super 2+) (sub 0.40) ) (Al (sub 0.39) Cr (sub 1.50) Fe (super 3+) (sub 0.09) )O (sub 4). Powder Diffraction: , Pages 96-99. 1998., 1998

Super-accuracy and super-resolution getting around the diffraction limit. Methods in Enzymology 475: 1-26, 2010

A three-camera imaging microscope for high-speed single-molecule tracking and super-resolution imaging in living cells. Proceedings of Spie--the International Society for Optical Engineering 9550(): 955008-955008, 2016

Resonant metalenses for breaking the diffraction barrier. Physical Review Letters 104(20): 203901-203901, 2010