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Cell-based antiviral screening against coronaviruses: developing virus-specific and broad-spectrum inhibitors



Cell-based antiviral screening against coronaviruses: developing virus-specific and broad-spectrum inhibitors



Antiviral Research 101: 105-112



To combat the public health threat from emerging coronaviruses (CoV), the development of antiviral therapies with either virus-specific or pan-coronaviral activities is necessary. An important step in antiviral drug development is the screening of potential inhibitors in cell-based systems. The recent emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) necessitates adapting methods that have been used to identify antivirals against severe acute respiratory syndrome coronavirus (SARS-CoV) and developing new approaches to more efficiently screen antiviral drugs. In this article we review cell-based assays using infectious virus (BSL-3) and surrogate assays (BSL-2) that can be implemented to accelerate antiviral development against MERS-CoV and future emergent coronaviruses. This paper forms part of a series of invited articles in Antiviral Research on "From SARS to MERS: 10years of research on highly pathogenic human coronaviruses."

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Accession: 051983299

Download citation: RISBibTeXText

PMID: 24269477

DOI: 10.1016/j.antiviral.2013.11.004


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