+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cell-based antiviral screening against coronaviruses: developing virus-specific and broad-spectrum inhibitors

Cell-based antiviral screening against coronaviruses: developing virus-specific and broad-spectrum inhibitors

Antiviral Research 101: 105-112

To combat the public health threat from emerging coronaviruses (CoV), the development of antiviral therapies with either virus-specific or pan-coronaviral activities is necessary. An important step in antiviral drug development is the screening of potential inhibitors in cell-based systems. The recent emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) necessitates adapting methods that have been used to identify antivirals against severe acute respiratory syndrome coronavirus (SARS-CoV) and developing new approaches to more efficiently screen antiviral drugs. In this article we review cell-based assays using infectious virus (BSL-3) and surrogate assays (BSL-2) that can be implemented to accelerate antiviral development against MERS-CoV and future emergent coronaviruses. This paper forms part of a series of invited articles in Antiviral Research on "From SARS to MERS: 10years of research on highly pathogenic human coronaviruses."

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 051983299

Download citation: RISBibTeXText

PMID: 24269477

DOI: 10.1016/j.antiviral.2013.11.004

Related references

High-Throughput Screening and Identification of Potent Broad-Spectrum Inhibitors of Coronaviruses. Journal of Virology 93(12):, 2019

Broad-spectrum antiviral GS-5734 inhibits both epidemic and zoonotic coronaviruses. Science Translational Medicine 9(396):, 2017

Computational screening of known broad-spectrum antiviral small organic molecules for potential influenza HA stem inhibitors. Plos one 13(9): E0203148, 2018

Screening Bioactives Reveals Nanchangmycin as a Broad Spectrum Antiviral Active against Zika Virus. Cell Reports 18(3): 804-815, 2017

A Novel Scaffold for Developing Specific or Broad-Spectrum Chitinase Inhibitors. Journal of Chemical Information and Modeling 56(12): 2413-2420, 2016

Cell-based antiviral assays for screening and profiling inhibitors against dengue virus. Methods in Molecular Biology 1030: 185-194, 2013

Mechanism-based covalent neuraminidase inhibitors with broad-spectrum influenza antiviral activity. Science 340(6128): 71-75, 2013

Optimization of Isothiazolo[4,3- b]pyridine-Based Inhibitors of Cyclin G Associated Kinase (GAK) with Broad-Spectrum Antiviral Activity. Journal of Medicinal Chemistry 61(14): 6178-6192, 2018

Development of intergenotypic chimeric replicons for broad-spectrum antiviral activity determination of hepatitis C virus polymerase inhibitors. 2007

Development of intergenotypic chimeric replicons to determine the broad-spectrum antiviral activities of hepatitis C virus polymerase inhibitors. Antimicrobial Agents and ChemoTherapy 52(10): 3523-3531, 2008

Comparative efficacy of broad-spectrum antiviral agents as inhibitors of African swine fever virus replication in vitro. Antiviral Research 7(3): 151-160, 1987

3C protease of enterovirus 68: structure-based design of Michael acceptor inhibitors and their broad-spectrum antiviral effects against picornaviruses. Journal of Virology 87(8): 4339-4351, 2013

Development of a replicon cell line-based high throughput antiviral assay for screening inhibitors of Zika virus. Antiviral Research 150: 148-154, 2018

Antiviral Activity of Broad-Spectrum and Enterovirus-Specific Inhibitors against Clinical Isolates of Enterovirus D68. Antimicrobial Agents and ChemoTherapy 59(12): 7782-7785, 2015

Profiling of substrate-specificity and rational design of broad-spectrum peptidomimetic inhibitors for main proteases of coronaviruses. Hong Kong Medical Journal 20(Suppl. 4): 22-25, 2014