EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Characterization of human immunodeficiency virus-1 (HIV-1) rev by (time-resolved) fluorescence spectroscopy


Characterization of human immunodeficiency virus-1 (HIV-1) rev by (time-resolved) fluorescence spectroscopy



Journal of Fluorescence 4(4): 299-302



ISSN/ISBN: 1053-0509

PMID: 24233603

DOI: 10.1007/bf01881444

Fluorescence spectroscopy has been applied to the single tryptophan-containing regulatory protein Rev of human immunodeficiency virus (HIV-1). The fluorescence emission was found to have a maximum at 336 nm which refers to a surrounding of the chromophore of intermediate polarity. Fluorescence transients recorded at the maximum of fluorescence were found to decay nonexponentially. A bimodal lifetime distribution is obtained from exponential series analysis (ESM) with centers at 1.7 and 4.5 ns. Two microenvironments for tryptophan are suggested to be responsible for the two lifetime distributions. No innerfilter effect occurred in a Rev solution up to a concentration of 40 μM. A data quality study of ESM analysis as function of collected counts in the peak channel maximum (CIM) showed that, for reliable reconvolution, at least 15,000 CIM are necessary. The widths of the two distributions are shown to be temperature dependent. The broadening of the lifetime distributions when the temperature is raised to 50°C is interpreted as extension of the number of conformational substates which do not interconvert on the fluorescence time scale. The thermal deactivation (temperature quenching) is reflected in a constant decrease in the center of the short-lived lifetime distribution.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052033485

Download citation: RISBibTeXText

Related references

Structural and dynamic characterization of the aromatic amino acids of the human immunodeficiency virus type I nucleocapsid protein zinc fingers and their involvement in heterologous tRNA(Phe) binding: a steady-state and time-resolved fluorescence study. Biophysical Journal 65(4): 1513-1522, 1993

Time-resolved fluorescence investigation of the human immunodeficiency virus type 1 nucleocapsid protein: influence of the binding of nucleic acids. Biophysical Journal 76(3): 1561-1570, 1999

Solid-phase time-resolved fluorescence detection of human immunodeficiency virus polymerase chain reaction amplification products. Analytical Biochemistry 202(1): 146-151, 1992

Detection of human immunodeficiency virus type 1 by using the polymerase chain reaction and a time-resolved fluorescence-based hybridization assay. Journal of Clinical Microbiology 29(4): 798-804, 1991

A continuous time-resolved fluorescence assay for human immunodeficiency virus reverse transcriptase to determine enzymatic mechanisms of resistance. Antiviral Research 46(1): A48, 2000

Novel Time-Resolved Fluorescence Europium Nanoparticle Immunoassay for Detection of Human Immunodeficiency Virus-1 Group O Viruses Using Microplate and Microchip Platforms. Aids Research and Human Retroviruses 32(6): 612-619, 2016

Photoinduced Cl transfer reaction between biphenyl and carbon tetrachloride studied by nanosecond time-resolved infrared spectroscopy and picosecond time-resolved fluorescence spectroscopy. Journal of Molecular Structure 598(1): 97-102, 2001

Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine. Molecules 22(1), 2016

Characterization of amino acid side chain dynamics in a zinc-finger peptide using carbon-13 NMR spectroscopy and time-resolved fluorescence spectroscopy. Journal of the American Chemical Society 115(14): 6333-6345, 1993

Photophysical and hydrodynamics characterization of yeast p13-suc1 by time resolved fluorescence spectroscopy. Biophysical Journal 68(2 Part 2): A190, 1995

Arterial lesions characterization by time-resolved laser-induced fluorescence spectroscopy. Photochemistry and Photobiology 69(SPEC ISSUE ): 69S, 1999

Resolution and characterization of tryptophyl fluorescence of hen egg-white lysozyme by quenching- and time-resolved spectroscopy. Bioscience Biotechnology and Biochemistry 63(2): 329-336, 1999

Application of the laguerre deconvolution method for time-resolved fluorescence spectroscopy to the characterization of atherosclerotic plaques. Conference Proceedings: .. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference 2005: 6559-6562, 2005

Characterization and detection of adulterated whey protein supplements using stationary and time-resolved fluorescence spectroscopy. Lwt 97: 180-186, 2018

Time-resolved fluorescence spectroscopy of hematoporphyrin-derivative in human lymphocytes. Chemico-Biological Interactions 50(2): 135-141, 1984