EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Characterization of nanostructured surfaces generated by reconstitution of the porin MspA from Mycobacterium smegmatis


Characterization of nanostructured surfaces generated by reconstitution of the porin MspA from Mycobacterium smegmatis



Small 3(6): 1084-1097



ISSN/ISBN: 1613-6810

PMID: 17514767

DOI: 10.1002/smll.200600559

Nanostructures with long-term stability at the surface of gold electrodes are generated by reconstituting the porin MspA from Mycobacterium smegmatis into a specially designed monolayer of long-chain lipid surfactant on gold. Tailored surface coverage of gold electrodes with long-chain surfactants is achieved by electrochemically assisted deposition of organic thiosulfates (Bunte salts). The subsequent reconstitution of the octameric-pore MspA is guided by its extraordinary self-assembling properties. Importantly, electrochemical reduction of copper(II) yields copper nanoparticles within the MspA nanopores. Electrochemical impedance spectroscopy, reflection electron microscopy, and atomic force microscopy (AFM) show that: 1) the MspA pores within the self-assembled monolayer (SAM) are monodisperse and electrochemically active, 2) MspA reconstitutes in SAMs and with a 10-nm thickness, 3) AFM is a suitable method to detect pores within SAMs, and 4) the electrochemical reduction of Cu2+ to Cu0 under overpotential conditions starts within the MspA pores.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052034887

Download citation: RISBibTeXText

Related references

Poly-N-Isopropylacrylamide/acrylic Acid Copolymers for the Generation of Nanostructures at Mica Surfaces and as Hydrophobic Host Systems for the Porin MspA from Mycobacterium smegmatis. Journal of Physical Chemistry. C Nanomaterials and Interfaces 113(37): 16485-16494, 2009

The porin MspA from Mycobacterium smegmatis improves growth of Mycobacterium bovis BCG. International Journal of Medical Microbiology: Ijmm 294(4): 235-245, 2004

Cloning of the mspA gene encoding a porin from Mycobacterium smegmatis. Molecular Microbiology 33(5): 933-945, 1999

Topology of the porin MspA in the outer membrane of Mycobacterium smegmatis. Journal of Biological Chemistry 281(9): 5908-5915, 2006

Functions of the periplasmic loop of the porin MspA from Mycobacterium smegmatis. Journal of Biological Chemistry 284(15): 10223-10231, 2009

Expression of the major porin gene mspA is regulated in Mycobacterium smegmatis. Journal of Bacteriology 189(3): 958-967, 2007

Tailored Polymeric Membranes for Mycobacterium Smegmatis Porin A (MspA) Based Biosensors. Journal of Materials Chemistry. B 3(25): 5080-5086, 2015

Allosteric Switching of Calmodulin in a Mycobacterium smegmatis porin A (MspA) Nanopore-Trap. Angewandte Chemie 60(44): 23863-23870, 2021

Mapping Potential Engineering Sites of Mycobacterium smegmatis porin a (MspA) to Form a Nanoreactor. Acs Sensors 6(6): 2449-2456, 2021

Single Molecule Ratcheting Motion of Peptides in a Mycobacterium smegmatis Porin a (MspA) Nanopore. Nano Letters 21(15): 6703-6710, 2021

Translocation Behaviors of Synthetic Polyelectrolytes through Alpha-Hemolysin (α-HL) and Mycobacterium smegmatis Porin a (MspA) Nanopores. Journal of the Electrochemical Society 169(5), 2022

A Single-Molecule Observation of Dichloroaurate(I) Binding to an Engineered Mycobacterium smegmatis porin a (MspA) Nanopore. Analytical Chemistry 93(3): 1529-1536, 2021

Rapid and multiplex preparation of engineered Mycobacterium smegmatis porin A (MspA) nanopores for single molecule sensing and sequencing. Chemical Science 12(27): 9339-9346, 2021

Machine Learning Assisted Simultaneous Structural Profiling of Differently Charged Proteins in a Mycobacterium smegmatis Porin a (MspA) Electroosmotic Trap. Journal of the American Chemical Society 144(2): 757-768, 2022

Single molecule observation of hard-soft-acid-base (HSAB) interaction in engineered Mycobacterium smegmatis porin A (MspA) nanopores. Chemical Science 11(3): 879-887, 2019