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Chloroquine differentially modulates inflammatory cytokine expression in RAW 264.7 cells in response to inactivated Staphylococcus aureus



Chloroquine differentially modulates inflammatory cytokine expression in RAW 264.7 cells in response to inactivated Staphylococcus aureus



Inflammation 38(2): 745-755



The anti-inflammatory property of chloroquine (CQ) has long been recognized. This study aimed to investigate the effect of CQ on proinflammatory cytokine expression in RAW 264.7 macrophages stimulated with heat-inactivated Staphylococcus aureus cells (SAC). The results showed that CQ treatment reduced the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and CC chemokine ligand 2 (CCL2) in culture medium but increased intracellular protein expression of TNF-α and CCL2 in SAC-activated cells. However, CQ showed differential effects on their messenger RNA (mRNA) levels: it reduced IL-6 mRNA expression, increased CCL2 mRNA levels, but had no effect on TNF-α mRNA. The SAC-activated inflammatory signaling pathways were slightly or minimally affected by CQ treatment. Additionally, CQ increased the accumulation of pro-TNF-α proteins within cells, suggesting an inhibition of pro-TNF-α processing and secretion. Collectively, CQ differentially modulated proinflammatory cytokine expression in Gram-positive bacterium-activated macrophages at multiple regulatory layers in the course of their biosynthesis.

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Accession: 052067345

Download citation: RISBibTeXText

PMID: 25085745

DOI: 10.1007/s10753-014-9985-z


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