+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution



Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution



Biophysical Journal 105(1): L01-L03



We report on a fiber laser-based stimulated emission-depletion microscope providing down to ∼20 nm resolution in raw data images as well as 15-19 nm diameter probing areas in fluorescence correlation spectroscopy. Stimulated emission depletion pulses of nanosecond duration and 775 nm wavelength are used to silence two fluorophores simultaneously, ensuring offset-free colocalization analysis. The versatility of this superresolution method is exemplified by revealing the octameric arrangement of Xenopus nuclear pore complexes and by quantifying the diffusion of labeled lipid molecules in artificial and living cell membranes.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052151650

Download citation: RISBibTeXText

PMID: 23823248

DOI: 10.1016/j.bpj.2013.05.029


Related references

Dual channel STED nanoscopy of lytic granules on actin filaments in natural killer cells. Communicative and Integrative Biology 5(2): 184-186, 2012

Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator. Plos One 7(6): E40003, 2013

Dual-label STED nanoscopy of living cells using photochromism. Nano Letters 11(9): 3970-3973, 2012

Increasing fluorescence lifetime for resolution improvement in STED nanoscopy. Journal of Biophotonics 2018: E201800315, 2018

2000-fold parallelized dual-color STED fluorescence nanoscopy. Optics Express 23(1): 211-223, 2015

Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy. Biophysical Journal 110(11): 2441-2450, 2017

Visualization of the immunological synapse by dual color time-gated stimulated emission depletion (STED) nanoscopy. Journal of Visualized Experiments 2014(85), 2014

STED nanoscopy reveals molecular details of cholesterol- and cytoskeleton-modulated lipid interactions in living cells. Biophysical Journal 101(7): 1651-1660, 2012

Direct and precise length measurement of single, stretched DNA fragments by dynamic molecular combing and STED nanoscopy. Analytical and Bioanalytical Chemistry 408(23): 6453-6459, 2018

Low-Saturation-Intensity, High-Photostability, and High-Resolution STED Nanoscopy Assisted by CsPbBr 3 Quantum Dots. Advanced Materials 30(23): E1800167, 2018

STED nanoscopy: a glimpse into the future. Cell and Tissue Research 360(1): 143-150, 2015

Parallelized STED fluorescence nanoscopy. Optics Express 19(24): 23716-23726, 2012

Adaptive-illumination STED nanoscopy. Proceedings of the National Academy of Sciences of the United States of America 114(37): 9797-9802, 2017

Dual-color STED microscopy at 30-nm focal-plane resolution. Small 4(8): 1095-1100, 2008

Dual channel RESOLFT nanoscopy by using fluorescent state kinetics. Nano Letters 15(1): 103-106, 2016