+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Collagen-binding protein, Aegyptin, regulates probing time and blood feeding success in the dengue vector mosquito, Aedes aegypti

Collagen-binding protein, Aegyptin, regulates probing time and blood feeding success in the dengue vector mosquito, Aedes aegypti

Proceedings of the National Academy of Sciences of the United States of America 111(19): 6946-6951

Mosquito salivary glands have important roles in blood feeding and pathogen transmission. However, the biological relevance of many salivary components has yet to be determined. Aegyptin, a secreted salivary protein from Aedes aegypti, binds collagen and inhibits platelet aggregation and adhesion. We used a transgenic approach to study the relevance of Aegyptin in mosquito blood feeding. Aedes aegypti manipulated genetically to express gene-specific inverted-repeat RNA sequences exhibited significant reductions in Aegyptin mRNA accumulation (85-87%) and protein levels (>80-fold) in female mosquito salivary glands. Transgenic mosquitoes had longer probing times (78-300 s, P < 0.0001) when feeding on mice compared with controls (15-56 s), feeding success was reduced, and those feeding took smaller blood meals. However, no differences in feeding success or blood meal size were found in membrane feeding experiments using defibrinated human blood. Salivary gland extracts from transgenic mosquitoes failed to inhibit collagen-induced platelet aggregation in vitro. Reductions of Aegyptin did not affect salivary ADP-induced platelet aggregation inhibition or disturb anticlotting activities. Our results demonstrate the relevance of Aegyptin for A. aegypti blood feeding, providing further support for the hypothesis that platelet aggregation inhibition is a vital salivary function in blood feeding arthropods. It has been suggested that the multiple mosquito salivary components mediating platelet aggregation (i.e., Aegyptin, apyrase, D7) represent functional redundancy. Our findings do not support this hypothesis; instead, they indicate that multiple salivary components work synergistically and are necessary to achieve maximum blood feeding efficiency.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 052165672

Download citation: RISBibTeXText

PMID: 24778255

DOI: 10.1073/pnas.1404179111

Related references

Wolbachia infection reduces blood-feeding success in the dengue fever mosquito, Aedes aegypti. Plos Neglected Tropical Diseases 3(9): E516, 2009

Blood meal induced microRNA regulates development and immune associated genes in the Dengue mosquito vector, Aedes aegypti. Insect Biochemistry and Molecular Biology 43(2): 146-152, 2013

Factors associated with male mating success of the dengue vector mosquito, Aedes aegypti. American Journal of Tropical Medicine and Hygiene 80(3): 395-400, 2009

Protein expression in the salivary glands of dengue-infected Aedes aegypti mosquitoes and blood-feeding success. Southeast Asian Journal of Tropical Medicine and Public Health 43(6): 1346-1357, 2012

Co-occurrence patterns of the dengue vector Aedes aegypti and Aedes mediovitattus, a dengue competent mosquito in Puerto Rico. Ecohealth 8(3): 365-375, 2011

Aedes aegypti salivary protein "aegyptin" co-inoculation modulates dengue virus infection in the vertebrate host. Virology 468-470: 133-139, 2014

Productivity and population density estimates of the dengue vector mosquito Aedes aegypti (Stegomyia aegypti) in Australia. Medical and Veterinary Entomology 27(3): 313-322, 2013

Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: potential tools for control of female feeding and reproduction. Insect Biochemistry and Molecular Biology 38(2): 176-189, 2008

Synthesis, depletion and cell-type expression of a protein from the male accessory glands of the dengue vector mosquito Aedes aegypti. Journal of Insect Physiology 70: 117-124, 2014

Glytube: a conical tube and parafilm M-based method as a simplified device to artificially blood-feed the dengue vector mosquito, Aedes aegypti. Plos one 8(1): E53816, 2013

Probing functional polymorphisms in the dengue vector, Aedes aegypti. Bmc Genomics 14: 739, 2013

Blood-feeding success of the mosquito Aedes aegypti depends on the transmission route of its parasite Edhazardia aedis. Oikos 78(2): 311-316, 1997

Larval competition between the introduced vector of dengue fever in australia aedes aegypti and a native container breeding mosquito aedes notoscriptus diptera culicidae. Australian Journal of Zoology 34(4): 527-534, 1986

Possible jungle dengue recent studies and hypotheses distribution human primary host mosquito vector virus aedes aegypti monkey aedes albopictus. Japanese Journal of Medical Science & Biology 20(SUPP): 69-74, 1967

Larval competition between the introduced vector of dengue fever in Australia, Aedes aegypti (L.), and a native container-breeding mosquito, Aedes notoscriptus (Skuse) (Diptera: Culicidae). Australian Journal of Zoology 344: 527-534, 1986