+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparative quantitative proteomic analysis of embryogenic and non-embryogenic calli in maize suggests the role of oxylipins in plant totipotency



Comparative quantitative proteomic analysis of embryogenic and non-embryogenic calli in maize suggests the role of oxylipins in plant totipotency



Journal of Proteomics 104: 57-65



Totipotency, the ability of somatic plant cell to generate whole plant through somatic embryogenesis, is still not well understood. In this study, maize immature zygotic embryos were used to generate embryogenic (EC) and non-embryogenic (NEC) calli. In order to compare proteomes of EC and NEC, two-dimensional electrophoresis (2-DE) in combination with mass spectrometry was used. This approach resulted into 361 quantified 2-DE spots out of which 44 were found statistically significantly differentially abundant between EC and NEC. Mass spectrometry provided the identity for 23 proteins that were classified into 8 metabolic categories. The most abundant were proteins associated with energy followed by proteins associated with disease and defense. Based on the abundances of identified proteins in this and other studies, working model for plant totipotency was proposed. One aspect of this working model suggests that increased abundances of proteins associated with pyruvate biosynthesis and suppression of embryogenic genes might be responsible for differences between EC and NEC cells. Furthermore we speculate that the increased abundance of lipoxygenase in the NEC cells results in changes in the equilibrium levels of one or more signaling molecules and is at least partly responsible for somatic cell reprogramming during totipotency. Totipotency, the ability of somatic plant cell to generate whole plant through somatic embryogenesis, is still not well understood. In order to further advance understanding of this biological phenomenon, proteomes of embryogenic and non-embryogenic callus, derived from immature zygotic embryos of inbred maize line A19, were compared using 2-DE based proteomic technology. Based on the abundances of identified proteins in this and other studies, working model for plant totipotency was proposed. One aspect of this working model suggests that increased abundances of proteins associated with pyruvate biosynthesis and suppression of embryogenic genes might be responsible for differences between EC and NEC cells. Furthermore we speculate that the increased abundance of lipoxygenase in the NEC cells results in changes in the equilibrium levels of one or more signaling molecules and is at least partly responsible for somatic cell reprogramming during totipotency. This article is part of a Special Issue entitled: Environmental and structural proteomics.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052216250

Download citation: RISBibTeXText

PMID: 24530378

DOI: 10.1016/j.jprot.2014.02.003


Related references

iTRAQ-Based Quantitative Proteomic Analysis of Embryogenic and Non-embryogenic Calli Derived from a Maize (Zea mays L.) Inbred Line Y423. International Journal of Molecular Sciences 19(12), 2018

Comparative proteomic analysis of the H99 inbred maize Zea mays L line in embryogenic and non-embryogenic callus during somatic embryogenesis. Plant Cell, Tissue and Organ Culture (PCTOC) 113(1): 103-119, 2013

iTRAQ-based comparative proteomic analysis of embryogenic and non-embryogenic tissues of Prince Rupprechts larch (Larix principis-rupprechtiiMayr). Plant Cell, Tissue and Organ Culture 120(2): 655-669, 2015

A comparative study of the biochemical metabolites in embryogenic and non-embryogenic calli of cotton cultivar Coker 201. Acta Agronomica Sinica 18(3): 176-182, 1992

Comparative anatomy of embryogenic and non-embryogenic calli from Pimpinella brachycarpa. Journal of Plant Biology 50(3): 344-350, 2007

Induction of embryogenic calli from caryopses of common bermudagrass and ultrastructure of embryogenic cells as well as plant regeneration. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao 30(2): 209-215, 2004

The effect of abscisic acid and sucrose on post-thaw embryogenic competence and subsequent plant recovery from embryogenic calli of cassava. American Eurasian Journal Of Agricultural And Environmental Science: 4, 663-671, 2008

Analysis of proteins from embryogenic and non embryogenic rice oryza sativa l. calli. Plant Science (Shannon) 48(3): 181-188, 1987

Rice embryogenic calli express a unique set of microRNAs, suggesting regulatory roles of microRNAs in plant post-embryogenic development. Febs Letters 580(21): 5111-5116, 2006

Comparison of embryogenic and non-embryogenic cotton calli for gene expression involved in regeneration of cotton plant. In Vitro Cellular & Developmental Biology Animal 35(3 PART 2): 44 A, March, 1999

Differentiated expression of VvSUC12 and VvSUC27 in embryogenic and non-embryogenic calli of Vitis vinifera L. Sheng Wu Gong Cheng Xue Bao 26(4): 530-537, 2011

Serological study on non-embryogenic and embryogenic calli of the European black pine and silver fir. Journal of Applied Botany 71(3-4): 108-110, 1997

Differences in invertase activity in embryogenic and non-embryogenic calli from Medicago arborea. Plant cell tissue and organ culture 67(2): 145-151, 2001

Endogenous cytokinin levels in embryogenic and non-embryogenic calli of Medicago arborea L. Plant Science 163(5): 955-960, 2002

Identification of differentially accumulated proteins associated with embryogenic and non-embryogenic calli in saffron (Crocus sativus L.). Proteome Science 10(1): 3, 2012