+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparison of Magnetic Intensities for Mesenchymal Stem Cell Targeting Therapy on Ischemic Myocardial Repair: High Magnetic Intensity Improves Cell Retention but Has no Additional Functional Benefit



Comparison of Magnetic Intensities for Mesenchymal Stem Cell Targeting Therapy on Ischemic Myocardial Repair: High Magnetic Intensity Improves Cell Retention but Has no Additional Functional Benefit



Cell Transplantation 24(10): 1981-1997



Magnetic targeting has the potential to enhance the therapeutic effects of stem cells through increasing retention of transplanted cells. To investigate the effects of magnetic targeting intensities on cell transplantation, we performed different magnetic intensities for mesenchymal stem cell (MSC)-targeting therapy in a rat model of ischemia/reperfusion. Rat MSCs labeled with superparamagnetic oxide nanoparticles (SPIOs) were injected into the left ventricular (LV) cavity of rats during a brief aorta and pulmonary artery occlusion. The 0.15 Tesla (T), 0.3 T, and 0.6 T magnets were placed 0∼1 mm above the injured myocardium during and after the injection of 1 × 10(6) MSCs. Fluorescence imaging and quantitative PCR revealed that magnetic targeting enhanced cell retention in the heart at 24 h in a magnetic field strength-dependent manner. Compared with the 0 T group, three magnetic targeting groups enhanced varying cell engraftment at 3 weeks, at which time LV remodeling was maximally attenuated, and the therapeutic benefit (LV ejection fraction) was also highest in the 0.3 T groups. Interestingly, due to the low MSC engraftment resulting from microvascular embolisms, the 0.6 T group failed to translate into additional therapeutic outcomes, though it had the highest cell retention. Magnetic targeting enhances cell retention in a magnetic field strength-dependent manner. However, too high of a magnetic intensity may result in microembolization and consequently undermine the functional benefits of cell transplantation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052226955

Download citation: RISBibTeXText

PMID: 25375750

DOI: 10.3727/096368914x685302


Related references

Magnetic enhancement of cell retention, engraftment, and functional benefit after intracoronary delivery of cardiac-derived stem cells in a rat model of ischemia/reperfusion. Cell Transplantation 21(6): 1121-1135, 2012

Mesenchymal Stem Cell Magnetization: Magnetic Multilayer Microcapsule Uptake, Toxicity, Impact on Functional Properties, and Perspectives for Magnetic Delivery. Advanced Healthcare Materials 5(24): 3182-3190, 2016

Autonomous magnetic labelling of functional mesenchymal stem cells for improved traceability and spatial control in cell therapy applications. Journal of Tissue Engineering and Regenerative Medicine 11(8): 2333-2348, 2017

Designing 3D Mesenchymal Stem Cell Sheets Merging Magnetic and Fluorescent Features: When Cell Sheet Technology Meets Image-Guided Cell Therapy. Theranostics 6(5): 739-751, 2016

In vitro cartilage formation using TGF-beta-immobilized magnetic beads and mesenchymal stem cell-magnetic bead complexes under magnetic field conditions. Journal of Biomedical Materials Research. Part a 92(1): 196-204, 2010

An in vitro model of mesenchymal stem cell targeting using magnetic particle labelling. Journal of Tissue Engineering and Regenerative Medicine 9(6): 724-733, 2015

Magnetic targeting enhances retrograde cell retention in a rat model of myocardial infarction. Stem Cell Research and Therapy 4(6): 149, 2013

Additional benefit of combined therapy with melatonin and apoptotic adipose-derived mesenchymal stem cell against sepsis-induced kidney injury. Journal of Pineal Research 57(1): 16-32, 2014

Chemokines in mesenchymal stem cell therapy for bone repair: a novel concept of recruiting mesenchymal stem cells and the possible cell sources. Modern Rheumatology 21(2): 113-121, 2011

Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles. Advances in Clinical and Experimental Medicine 26(8): 1171-1178, 2017

The safety and efficacy of magnetic targeting using autologous mesenchymal stem cells for cartilage repair. Knee Surgery Sports Traumatology Arthroscopy 26(12): 3626-3635, 2018

Magnetic targeting of smooth muscle cells in vitro using a magnetic bacterial cellulose to improve cell retention in tissue-engineering vascular grafts. Acta Biomaterialia 77: 172-181, 2018

Magnetic field application or mechanical stimulation via magnetic microparticles does not enhance chondrogenesis in mesenchymal stem cell sheets. Biomaterials Science 5(7): 1241-1245, 2017

VEGF overexpression improves mesenchymal stem cell sheet transplantation therapy for acute myocardial infarction. Journal of Tissue Engineering and Regenerative Medicine aop(aop): 0-0, 2012

Modification of Mesenchymal Stem Cells as a way to Improve the Effectiveness of Cell Therapy of Ischemic Myocardial Injury. Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova 101(9): 985-998, 2015