+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Comparison of a novel whole blood transglutaminase-based ELISA with a whole blood rapid antibody test and established conventional serological celiac disease assays

Comparison of a novel whole blood transglutaminase-based ELISA with a whole blood rapid antibody test and established conventional serological celiac disease assays

Journal of Pediatric Gastroenterology and Nutrition 47(5): 562-567

Serum immunoglobulin A-class tissue transglutaminase (tTG-ab) and endomysial antibody (EMA) tests play a key role in the diagnostic evaluation of celiac disease. Recently, a novel whole blood rapid test based on self-tissue transglutaminase (tTG) was developed for celiac disease case finding. Based on the same principle, a whole blood self-tTG enzyme-linked immunosorbent assay (ELISA), especially applicable to large-scale screening of celiac disease, has been produced. We assessed the value of this new test in celiac disease antibody detection. The new test uses endogenous tTG found in red blood cells of whole blood in IgA-class tTG-ab measurement by detecting tTG-tTG-ab complexes formed after hemolysis of the whole blood sample. Stored whole blood samples from 150 untreated celiac disease patients and 107 control individuals without celiac disease were evaluated, and the test results were compared with those of the whole blood rapid test, 2 conventional serum-based tTG-ab ELISA tests, and 2 EMA tests. A total of 15 whole blood samples were found to be clotted or dried after storage and were excluded from further evaluation. The whole blood ELISA test had a specificity (98%) comparable to that of the conventional serological tests, the sensitivity (91%) being slightly lower. The test was concordant with the whole blood rapid test in 92% of cases, with 2 serological ELISA tests in 91% and 94% of cases and with EMA tests in 94% and 93% of cases. Whole blood self-tTG-based testing is accurate in celiac antibody detection, also when an ELISA method is applied. The testing requires no serum separation or external tTG.

(PDF emailed within 0-6 h: $19.90)

Accession: 052228037

Download citation: RISBibTeXText

PMID: 18979578

DOI: 10.1097/mpg.0b013e3181615cde

Related references

Measurement of streptokinase antibodies or inhibitors in blood Comparison of a rapid desk top test panel as conventional antibody and resistance assays. European Heart Journal 17(ABSTR SUPPL ): 203, 1996

Comparison of tissue transglutaminase-specific antibody assays with established antibody measurements for coeliac disease. Journal of Autoimmunity 12(1): 51-56, 1999

High prevalence of celiac disease in Brazilian blood donor volunteers based on screening by IgA antitissue transglutaminase antibody. European Journal of Gastroenterology & Hepatology 19(1): 43-49, 2007

Correlation of the Blood Test Results Obtained between Assays Using Microliter-scale Fingertip Blood Samples Collected with a Novel Blood Collection Device and Conventional Venous Blood Assays: a Secondary Publication in English. Keio Journal of Medicine, 2017

Field comparison of OraQuick ADVANCE Rapid HIV-1/2 antibody test and two blood-based rapid HIV antibody tests in Zambia. Bmc Infectious Diseases 12: 183-183, 2013

Solid-phase ELISA for tissue transglutaminase, an endomysial target for possible serological diagnosis of celiac disease. Canadian Journal of Gastroenterology 12(5): 323-324, 1998

Celiac disease could be a frequent disease in Mexico: prevalence of tissue transglutaminase antibody in healthy blood donors. Journal of Clinical Gastroenterology 40(8): 697-700, 2006

Strongly positive tissue transglutaminase antibody assays without celiac disease. Canadian Journal of Gastroenterology 18(1): 25-28, 2004

Anti-tissue transglutaminase antibody ELISA for accurate diagnosis of celiac disease. FASEB Journal 14(6): A1235, April 20, 2000

Comparative evaluation of a rapid diagnostic test, an antibody ELISA, and a pLDH ELISA in detecting asymptomatic malaria parasitaemia in blood donors in Buea, Cameroon. Infectious Diseases of Poverty 6(1): 103-103, 2017

Determination of anti-endomysium IgA antibodies in the diagnosis of celiac disease: comparison of a novel ELISA-based assay with conventional immunofluorescence. World Journal of Gastroenterology 12(17): 2779-2780, 2006

Comparison of an indirect fluorescent antibody test with an elisa for the serological studies of lyme disease. Abstracts of the Annual Meeting of the American Society for Microbiology 89: 487, 1989

Diagnostic accuracy of ten second-generation (human) tissue transglutaminase antibody assays in celiac disease. Clinical Chemistry 50(11): 2125-2135, 2004

Improved method for serological testing in celiac disease--IgA anti-endomysium antibody test: a comparison between monkey oesophagus and human umbilical cord as substrate in indirect immunofluorescence test. Scandinavian Journal of Clinical and Laboratory Investigation 58(7): 547-554, 1999

Diagnostic accuracy of IgA anti-tissue transglutaminase antibody assays in celiac disease patients with selective IgA deficiency. Annals of the New York Academy of Sciences 1109: 212-220, 2007