+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparison of telomere length and association with progenitor cell markers in lacrimal gland between Sjögren syndrome and non-Sjögren syndrome dry eye patients

Comparison of telomere length and association with progenitor cell markers in lacrimal gland between Sjögren syndrome and non-Sjögren syndrome dry eye patients

Molecular Vision 17: 1397-1404

Indicators of aging such as disruption of telomeric function due to shortening may be more frequent in dysfunctional lacrimal gland. The aims of this study were to 1) determine the viability of quantitative fluorescence in situ hybridization of telomeres (telo-FISH) for the assessment of telomere length in lacrimal gland in Sjögren and non- Sjögren syndrome patients; and 2) investigate the relationship between progenitor cell markers and telomere length in both groups. Quantitative fluorescence in situ hybridization with a peptide nucleic acid probe complementary to the telomere repeat sequence was performed on frozen sections from human lacrimal gland tissues. The mean fluorescence intensity of telomere spots was automatically quantified by image analysis as relative telomere length in lacrimal gland epithelial cells. Immunostaining for p63, nucleostemin, ATP-binding cassette, sub-family G, member 2 (ABCG2), and nestin was also performed. Telomere intensity in the Sjögren syndrome group (6,785.0±455) was significantly lower than that in the non-Sjögren syndrome group (7,494.7±477; p=0.02). Among the samples from the non-Sjögren syndrome group, immunostaining revealed that p63 was expressed in 1-3 acinar cells in each acinar unit and continuously in the basal layer of duct cells. In contrast, in the Sjögren syndrome group, p63 and nucleostemin showed a lower level of expression. ABCG2 was expressed in acinar cells in both sjogren and non-Sjogren syndrome. The results of this study indicate that 1) telo-FISH is a viable method of assessing telomere length in lacrimal gland, and 2) telomere length in Sjögren syndrome is shorter and associated with lower levels of expression of p63 and nucleostemin than in non-Sjögren syndrome.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 052244287

Download citation: RISBibTeXText

PMID: 21655359

Related references

The EULAR Sjogren's syndrome patient reported index as an independent determinant of health-related quality of life in primary Sjogren's syndrome patients: in comparison with non-Sjogren's sicca patients. Rheumatology 52(12): 2208-2217, 2014

Comparison of Meibomian Gland Imaging Findings and Lipid Layer Thickness between Primary Sjögren Syndrome and Non-Sjögren Syndrome Dry Eyes. Ocular Immunology and Inflammation 2019: 1-6, 2019

Chemokine saliva levels in patients with primary Sjögren's syndrome, associated Sjögren's syndrome, pre-clinical Sjögren's syndrome and systemic autoimmune diseases. Rheumatology 50(7): 1288-1292, 2012

Salivary gland and peripheral blood T helper 1 and 2 cell activity in Sjögren's syndrome compared with non-Sjögren's sicca syndrome. Annals of the Rheumatic Diseases 64(10): 1474-1479, 2005

Mononuclear cell phenotypes and immunoglobulin gene rearrangements in lacrimal gland biopsies from patients with Sjögren's syndrome. Ophthalmology 97(12): 1599-1605, 1990

Comparison of lacrimal and salivary gland involvement in Sjögren's syndrome. Archives of Otolaryngology--Head and Neck Surgery 129(9): 966-971, 2003

Cytokine concentrations in stimulated whole saliva among patients with primary Sjögren's syndrome, secondary Sjögren's syndrome, and patients with primary Sjögren's syndrome receiving varying doses of interferon for symptomatic treatment of the condition: a preliminary study. Clinical Oral Investigations 5(2): 133-135, 2001

Salivary gland scintigraphy in Sjogren's syndrome and patients with sicca symptoms but without Sjogren's syndrome: The psychological profiles and predictors for salivary gland dysfunction. Annals of the Rheumatic Diseases 62(10): 964-968, 2003

Detection of ebv in lacrimal gland biopsies of sjogren syndrome patients. Journal of Cellular Biochemistry Suppl. (13 PART E): 279, 1989

The reaction of the lacrimal gland to parasympathicomimetics in patients with Sjogren's syndrome. Ophthalmologica. Journal International d'Ophtalmologie. International Journal of Ophthalmology. Zeitschrift für Augenheilkunde 141: 51-52, 1961

Phenotypic features and predictors of the clinical severity of keratoconjunctivitis sicca and salivary gland dysfunction in patients with Sjögren's syndrome: a longitudinal analysis of the Korean Initiative of primary Sjögren's Syndrome (KISS) cohort. Scandinavian Journal of Rheumatology 2018: 1-9, 2018

Lacrimal gland accumulation of 67Ga-citrate in patients with Sjögren's syndrome. European Journal of Nuclear Medicine 9(5): 233-236, 1984

Manifestation of meibomian gland dysfunction in patients with Sjögren's syndrome, non-Sjögren's dry eye, and non-dry eye controls. International Ophthalmology 38(3): 1161-1167, 2017

Autoantibodies against lacrimal gland M3 muscarinic acetylcholine receptors in patients with primary Sjogren's syndrome. IOVS 39(1): 151-156, 1998

Comparison in T- and B-cell markers in patients with Sjögren's syndrome and systemic lupus erythematosus. Clinical Immunology and Immunopathology 22(2): 270-278, 1982