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Complete saccharification of cellulose at high temperature using endocellulase and beta-glucosidase from Pyrococcus sp

Complete saccharification of cellulose at high temperature using endocellulase and beta-glucosidase from Pyrococcus sp

Journal of Microbiology and Biotechnology 20(5): 889-892

We investigated a potential for glucose production from cellulose material using two kinds of hyperthermophilic enzymes, endo-cellulase (EG) and beta-glucosidase (BGL). Two BGLs from hyperthermophile Pyrococcus furiosus and mesophile Aspergillus aculeatus were compared for complete hydrolysis of cellulose with P. horikoshii endo-cellulase (EGPh). The combination reactions by each BGL enzyme and EGPh could produce only glucose without the other oligosaccharides from phosphoric acid swollen Avicel (PSA). The combination of the both hyperthermophilic cellulases, BGLPf and EGPh, will be adaptable to high efficient system to produce glucose at high temperature.

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Accession: 052260407

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PMID: 20519912

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