+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Complex assembly, crystallization and preliminary X-ray crystallographic studies of the swine major histocompatibility complex molecule SLA-1*1502



Complex assembly, crystallization and preliminary X-ray crystallographic studies of the swine major histocompatibility complex molecule SLA-1*1502



Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 5): 568-571



In order to illustrate the structure of the swine MHC class I (SLA-I) molecule and to evaluate the cytotoxic T lymphocyte (CTL) response against porcine reproductive and respiratory syndrome virus (PRRSV), the ternary complex of the SLA-I molecule termed SLA-1*1502 with β(2)-microglobulin and the CTL epitope TMPPGFELY (PRRSV-NSP9(TY9)) derived from PRRSV nonstructural protein 9 (residues 198-206) was assembled and crystallized. The crystal diffracted X-rays to 2.2 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 66.1, b = 74.1, c = 98.6 Å; it contained one molecule in the asymmetric unit. The Matthews coefficient and the solvent content were calculated to be 2.74 Å(3) Da(-1) and 55.17%, respectively. The results will be helpful in obtaining insight into the structural basis of the presentation of viral epitopes by SLA-I.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052261365

Download citation: RISBibTeXText

PMID: 21543863

DOI: 10.1107/s174430911100741x


Related references

Complex assembly, crystallization and preliminary X-ray crystallographic studies of duck MHC class I molecule. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 66(Pt 1): 99, 2010

Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule BF2*1501. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 69(Pt 2): 122-125, 2013

Complex assembly, crystallization and preliminary X-ray crystallographic studies of MHC H-2Kd complexed with an HBV-core nonapeptide. Acta Crystallographica. Section D Biological Crystallography 60(Pt 8): 1473-1475, 2004

Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the chicken CD8αα-BF2*0401 complex. Acta Crystallographica. Section F Structural Biology Communications 70(Pt 9): 1264-1267, 2014

Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod-Zwilch-ZW10 (RZZ) complex. Acta Crystallographica. Section F Structural Biology Communications 71(Pt 4): 438-442, 2015

Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the bovine CD8αα-BoLA-2*02201 complex. Acta Crystallographica. Section F Structural Biology Communications 70(Pt 6): 742-746, 2014

Complex assembly, crystallization and preliminary X-ray crystallographic studies of rhesus macaque MHC Mamu-A*01 complexed with an immunodominant SIV-Gag nonapeptide. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 61(Pt 6): 614-616, 2005

Complex assembly, crystallization and preliminary X- ray crystallographic studies of rhesus macaque MHC Mamu-A*01 complexed with an immunodominant SIV-Gag nonapeptide. Acta Crystallographica Section F Structural Biology and Crystallization Communications 61(Part 6): 614-616, 2005

Crystallization and preliminary X-ray crystallographic analysis of ribosome assembly factors: the Rpf2-Rrs1 complex. Acta Crystallographica. Section F Structural Biology Communications 70(Pt 12): 1649-1652, 2014

Crystallization and preliminary crystallographic analysis of the catalytic domain of human flap endonuclease 1 in complex with a nicked DNA product: use of a DPCS kit for efficient protein-DNA complex crystallization. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 64(Pt 1): 39-43, 2008

Stoichiometric tapasin interactions in the catalysis of major histocompatibility complex class I molecule assembly. Immunology 114(3): 346-353, 2005

Crystallization and preliminary X-ray crystallographic studies of transglutaminase 2 in complex with Ca2+. Acta Crystallographica. Section F Structural Biology Communications 70(Pt 4): 513-516, 2014

Crystallization and preliminary crystallographic studies of human RIG-I in complex with double-stranded RNA. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 65(Pt 6): 648-650, 2009

Crystallization and preliminary X-ray crystallographic studies of the Z-DNA-binding domain of a PKR-like kinase (PKZ) in complex with Z-DNA. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 65(Pt 3): 267-270, 2009

Crystallization and preliminary crystallographic studies of the YafN-YafO complex from Escherichia coli. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 68(Pt 8): 894-897, 2012