+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Compound evolutionary history of the rhesus macaque MHC class I B region revealed by microsatellite analysis and localization of retroviral sequences

Compound evolutionary history of the rhesus macaque MHC class I B region revealed by microsatellite analysis and localization of retroviral sequences

Plos One 4(1): E4287

In humans, the single polymorphic B locus of the major histocompatibility complex is linked to the microsatellite MIB. In rhesus macaques, however, haplotypes are characterized by the presence of unique combinations of multiple B genes, which may display different levels of polymorphism. The aim of the study was to shed light on the evolutionary history of this highly complex region. First, the robustness of the microsatellite MIB-linked to almost half of the B genes in rhesus macaques (Mamu-B)-for accurate B haplotyping was studied. Based on the physical map of an established haplotype comprising 7 MIB loci, each located next to a certain Mamu-B gene, two MIB loci, MIB1 and MIB6, were investigated in a panel of MHC homozygous monkeys. MIB1 revealed a complex genotyping pattern, whereas MIB6 analysis resulted in the detection of one or no amplicon. Both patterns are specific for a given B haplotype, show Mendelian segregation, and even allow a more precise haplotype definition than do traditional typing methods. Second, a search was performed for retroelements that may have played a role in duplication processes as observed in the macaque B region. This resulted in the description of two types of duplicons. One basic unit comprises an expressed Mamu-B gene, adjacent to an HERV16 copy closely linked to MIB. The second type of duplicon comprises a Mamu-B (pseudo)gene, linked to a truncated HERV16 structure lacking its MIB segment. Such truncation seems to coincide with the loss of B gene transcription. Subsequent to the duplication processes, recombination between MIB and Mamu-B loci appears to have occurred, resulting in a hyperplastic B region. Thus, analysis of MIB in addition to B loci allows deciphering of the compound evolutionary history of the class I B region in Old World monkeys.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 052269044

Download citation: RISBibTeXText

PMID: 19172173

DOI: 10.1371/journal.pone.0004287

Related references

Comparative and evolutionary analysis of the rhesus macaque extended MHC class II region. Immunogenetics 54(10): 699-704, 2003

Population analysis of the rhesus macaque class II region Unprecedented DR region polymorphism as compared to humans. Human Immunology 60(SUPPL 1): S26, 1999

The evolutionary history of the sinica-group of macaque monkeys as revealed by mtDNA restriction site analysis. Molecular Phylogenetics & Evolution 1(3): 215-222, 1992

Synthesis and analysis of a 640-bp pol region of novel human endogenous retroviral sequences and their evolutionary relationships. Virology 217(1): 1-10, 1996

Construction of sequence-ready map and genome sequencing analysis of rhesus macaque MHC class I region. Tissue Antigens 59(2 Supplement): 7-8, 2002

Microsatellite typing of the rhesus macaque MHC region. Immunogenetics 57(3-4): 198-209, 2005

Evolutionary stability of MHC class II haplotypes in different rhesus macaque populations. Genes & Immunity 4(Supplement 1): S2, 2003

Evolutionary stability of MHC class II haplotypes in diverse rhesus macaque populations. Immunogenetics 55(8): 540-551, 2003

Genetic variation and evolutionary demography of Fenneropenaeus chinensis populations, as revealed by the analysis of mitochondrial control region sequences. Genetics and Molecular Biology 33(2): 379-389, 2010

Genetic analysis of Toll/Interleukin-1 Receptor (TIR) domain sequences from rhesus macaque Toll-like receptors (TLRs) 1-10 reveals high homology to human TLR/TIR sequences. Immunogenetics 56(9): 667-674, 2004

Identification of residues within the L2 region of rhesus TRIM5alpha that are required for retroviral restriction and cytoplasmic body localization. Virology 405(1): 259-266, 2010

Peripheral blood integration site analysis in a rhesus macaque model of highly efficient retroviral ex vivo transduction of hematopoietic repopulating cells. Blood 94(10 SUPPL 1 PART 1): 671a, Nov 15, 1999

Evolutionary history of the genus Rhogeessa Chiroptera Vespertilionidae as revealed by mitochondrial DNA sequences. Journal of Mammalogy 89(3): 744-754, 2008