+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Compound-specific δ15N amino acid measurements in littoral mussels in the California upwelling ecosystem: a new approach to generating baseline δ15N Isoscapes for coastal ecosystems



Compound-specific δ15N amino acid measurements in littoral mussels in the California upwelling ecosystem: a new approach to generating baseline δ15N Isoscapes for coastal ecosystems



Plos One 9(6): E98087



We explored δ(15)N compound-specific amino acid isotope data (CSI-AA) in filter-feeding intertidal mussels (Mytilus californianus) as a new approach to construct integrated isoscapes of coastal primary production. We examined spatial δ(15)N gradients in the California Upwelling Ecosystem (CUE), determining bulk δ(15)N values of mussel tissue from 28 sites between Port Orford, Oregon and La Jolla, California, and applying CSI-AA at selected sites to decouple trophic effects from isotopic values at the base of the food web. Bulk δ(15)N values showed a strong linear trend with latitude, increasing from North to South (from ∼ 7‰ to ∼ 12‰, R(2) = 0.759). In contrast, CSI-AA trophic position estimates showed no correlation with latitude. The δ(15)N trend is therefore most consistent with a baseline δ(15)N gradient, likely due to the mixing of two source waters: low δ(15)N nitrate from the southward flowing surface California Current, and the northward transport of the California Undercurrent (CUC), with (15)N-enriched nitrate. This interpretation is strongly supported by a similar linear gradient in δ(15)N values of phenylalanine (δ(15)NPhe), the best AA proxy for baseline δ(15)N values. We hypothesize δ(15)N(Phe) values in intertidal mussels can approximate annual integrated δ(15)N values of coastal phytoplankton primary production. We therefore used δ(15)N(Phe) values to generate the first compound-specific nitrogen isoscape for the coastal Northeast Pacific, which indicates a remarkably linear gradient in coastal primary production δ(15)N values. We propose that δ(15)N(Phe) isoscapes derived from filter feeders can directly characterize baseline δ(15)N values across major biochemical provinces, with potential applications for understanding migratory and feeding patterns of top predators, monitoring effects of climate change, and study of paleo- archives.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052269275

Download citation: RISBibTeXText

PMID: 24887109

DOI: 10.1371/journal.pone.0098087


Related references

Practical considerations in the determination of compound-specific amino acid δ15N values in animal and plant tissues by gas chromatography-combustion-isotope ratio mass spectrometry, following derivatisation to their N-acetylisopropyl esters. Rapid Communications in Mass Spectrometry 26(19): 2328-2334, 2012

Compound-specific δ13C and δ15N analysis of amino acids: a rapid, chloroformate-based method for ecological studies. Rapid Communications in Mass Spectrometry 28(1): 96-108, 2014

Compound-Specific δ¹⁵N and δ¹³C Analyses of Amino Acids for Potential Discrimination between Organically and Conventionally Grown Wheat. Journal of Agricultural and Food Chemistry 63(25): 5841-5850, 2015

Declining foliar and litter δ¹⁵N diverge from soil, epiphyte and input δ¹⁵N along a 120,000 yr temperate rainforest chronosequence. New Phytologist 190(4): 941-952, 2011

Amino acid-specific δ 15 N trophic enrichment factors in fish fed with formulated diets varying in protein quantity and quality. Ecology and Evolution 8(18): 9192-9217, 2018

Reliability of isotopic fractionation (Δ15N, Δ13C) for the delimitation of trophic levels of oribatid mites: Diet strongly affects Δ13C but not Δ15N. Soil Biology and Biochemistry 101: 124-129, 2016

Nutrient regime shift in the western North Atlantic indicated by compound-specific δ15N of deep-sea gorgonian corals. Proceedings of the National Academy of Sciences of the United States of America 108(3): 1011-1015, 2011

Broad-scale patterns of tissue-δ15N and tissue-N indices in frondose Ulva spp.; developing a national baseline indicator of nitrogen-loading for coastal New Zealand. Marine Pollution Bulletin 67(1-2): 203-216, 2013

Cereal grain, rachis and pulse seed amino acid δ15N values as indicators of plant nitrogen metabolism. Phytochemistry 97: 20-29, 2014

Multi-Isotopic (δ2H, δ13C, δ15N) Tracing of Molt Origin for Red-Winged Blackbirds Associated with Agro-Ecosystems. Plos One 11(11): E0165996, 2017

New Insights on the Nitrogen Footprint of a Coastal Megalopolis from Coral-Hosted Symbiodinium δ 15 N. Environmental Science and Technology 51(4): 1981-1987, 2017

Patterns of Phosphorus, Nitrogen and Δ15N along a Peat Development Gradient in a Coastal Mire, Panama. Journal of Tropical Ecology 23(6): 683-691, 2007

Differential patterns of nitrogen and δ 15 N in soil and foliar along two urbanized rivers in a subtropical coastal city of southern China. Environmental Pollution 244: 907-914, 2018

The use of δ¹⁵N signatures of translocated macroalgae to map coastal nutrient plumes: improving species selection and spatial analysis of metropolitan datasets. Journal of Environmental Monitoring 14(9): 2399-2410, 2012

Stable isotope tracking of endangered sea turtles: validation with satellite telemetry and δ15N analysis of amino acids. Plos One 7(5): E37403, 2012