+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Compounds from Lactobacillus plantarum culture supernatants with potential pro-healing and anti-pathogenic properties in skin chronic wounds



Compounds from Lactobacillus plantarum culture supernatants with potential pro-healing and anti-pathogenic properties in skin chronic wounds



Pharmaceutical Biology 53(3): 350-358



It is necessary to advance the field of alternative treatments for chronic wounds that are financially accessible to the least economically developed countries. Previously we demonstrated that topical applications of Lactobacillus plantarum culture supernatants (LAPS) on human-infected chronic wounds reduce the pathogenic bioburden, the amount of necrotic tissue, and the wound area, as well as promote debridement, granulation tissue, and wound healing. To study LAPS chemically and biologically and to find potential molecules responsible for its pro-healing and anti-pathogenic properties in chronic wounds. (1) Chemical analysis: extracts were subjected to a column chromatography and the fractions obtained were studied by GCMS. (2) Quantification: dl-lactic acid (commercial kit), phenolic compounds (Folin-Ciocalteu), H2O2 (micro-titration), and cations (flame photometry). (3) Biological analysis: autoinducers type 2 (AI-2) (Vibrio harveyi BB170 bioassay), DNAase activity (Agar DNAase), and Pseudomonas aeruginosa biofilm inhibition (crystal violet technique). According to its biological activity, the most significant molecules found by GCMS were the following: antimicrobials (mevalonolactone, 5-methyl-hydantoine, benzoic acid, etc.); surfactants (di-palmitin, distearin, and 1,5-monolinolein); anesthetics (barbituric acid derivatives), and AI-2 precursors (4,5-dihydroxy-2,3-pentanedione and 2-methyl-2,3,3,4-tetrahydroxytetrahydrofurane). Concentrations measured (µg/mL): DL-lactic acid (11.71 ± 1.53) and H2O2 (36 ± 2.0); phenolic compounds (485.2 ± 15.20); sodium (370 ± 17); potassium 920 ± 24); calcium (20 ± 4); and magnesium (15 ± 3). DNAase from LAPS had activity on genomic DNA from PMNs and P. aeruginosa. The molecules and biological activities found in LAPS could explain the observed effects in human chronic wounds.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052269413

Download citation: RISBibTeXText

PMID: 25347359

DOI: 10.3109/13880209.2014.920037


Related references

Antipathogenic properties of Lactobacillus plantarum on Pseudomonas aeruginosa: the potential use of its supernatants in the treatment of infected chronic wounds. Wound Repair and Regeneration 20(4): 552-562, 2012

Lactobacillus plantarum culture supernatants improve intestinal tissue exposed to deoxynivalenol. Experimental and Toxicologic Pathology 69(8): 666-671, 2017

Culture supernatants from lactobacillus plantarum induce necrosis on a human promyelocytic leukemia cell line. Endocrine, Metabolic & Immune Disorders Drug Targets 13(2): 195-203, 2014

Culture Supernatants from Lactobacillus plantarum Induce Necrosis on a Human Promyelocytic Leukemia Cell Line. Proceedings of the Nutrition Society 72(OCE1): E78, 2013

Culture Supernatants from Lactobacillus plantarum Induce Necrosis on a Human Promyelocytic Leukemia Cell Line. Proceedings of the Nutrition Society 72(OCE1): E78, 2013

Anti-pathogenic and probiotic attributes of Lactobacillus salivarius and Lactobacillus plantarum strains isolated from feces of Algerian infants and adults. Research in Microbiology 168(3): 244-254, 2017

In vitro evaluation of the fermentation properties and potential probiotic activity of Lactobacillus plantarum C4 in batch culture systems. Lebensmittel-Wissenschaft Und-Technologie / Food Science and Technology 60(1): 420-426, 2015

Sensitivity of certain isolated lactobacilli of the human micro flora to antibiotics lactobacillus acidophilus lactobacillus plantarum lactobacillus casei lactobacillus fermenti lactobacillus brevis penicillin anti infect streptomycin anti infect oxytetracycline anti infect neomycin anti infect. Antibiotiki (moscow): 426-429, 1969

Cell-free supernatants obtained from fermentation of cheese whey hydrolyzates and phenylpyruvic acid by Lactobacillus plantarum as a source of antimicrobial compounds, bacteriocins, and natural aromas. Applied Biochemistry and Biotechnology 171(4): 1042-1060, 2014

Wound healing and angiogenic properties of supernatants from Lactobacillus cultures. Experimental Biology and Medicine 228(11): 1329-1337, 2003

Mesenchymal stem cells: potential for therapy and treatment of chronic non-healing skin wounds. Organogenesis 11(4): 183-206, 2015

Anti-Inflammatory and Anti-Oxidative Activities of Phenolic Compounds from Alnus sibirica Stems Fermented by Lactobacillus plantarum subsp. argentoratensis. Molecules 22(9): -, 2017

The Effect of Lactobacillus plantarum ATCC 8014 and Lactobacillus acidophilus NCFM Fermentation on Antioxidant Properties of Selected in Vitro Sprout Culture of Orthosiphon aristatus (Java Tea) as a Model Study. Antioxidants 1(1): 4-32, 2012

Antimicrobial, anti-adhesive and anti-biofilm potential of biosurfactants isolated from Pediococcus acidilactici and Lactobacillus plantarum against Staphylococcus aureus CMCC26003. Microbial Pathogenesis 127: 12-20, 2018

Anti-Candida activity of spent culture filtrate of Lactobacillus plantarum strain LR/14. Journal de Mycologie Medicale 24(2): E25-E34, 2015