+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Compounds from silicones alter enzyme activity in curing barnacle glue and model enzymes



Compounds from silicones alter enzyme activity in curing barnacle glue and model enzymes



Plos One 6(2): E16487



Attachment strength of fouling organisms on silicone coatings is low. We hypothesized that low attachment strength on silicones is, in part, due to the interaction of surface available components with natural glues. Components could alter curing of glues through bulk changes or specifically through altered enzyme activity. GC-MS analysis of silicone coatings showed surface-available siloxanes when the coatings were gently rubbed with a cotton swab for 15 seconds or given a 30 second rinse with methanol. Mixtures of compounds were found on 2 commercial and 8 model silicone coatings. The hypothesis that silicone components alter glue curing enzymes was tested with curing barnacle glue and with commercial enzymes. In our model, barnacle glue curing involves trypsin-like serine protease(s), which activate enzymes and structural proteins, and a transglutaminase which cross-links glue proteins. Transglutaminase activity was significantly altered upon exposure of curing glue from individual barnacles to silicone eluates. Activity of purified trypsin and, to a greater extent, transglutaminase was significantly altered by relevant concentrations of silicone polymer constituents. Surface-associated silicone compounds can disrupt glue curing and alter enzyme properties. Altered curing of natural glues has potential in fouling management.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 052269421

Download citation: RISBibTeXText

PMID: 21379573


Related references

Diverse clinical compounds alter the quaternary structure and inhibit the activity of an essential enzyme. Chemmedchem 6(6): 1067-1073, 2011

Chemical studies on the curing of domestic type tobacco plants. 1. Enzyme activity in primed leaves during air-curing. Bulletin of the Utsunomiya Tobacco Experiment Station (10): 73-85, 1971

Curing of glue by means of electrically heated aluminium foils placed in the glue-lines (gluing of laminated beams). Drev. Vyskum. 1, 25-44, 1969

Design and synthesis of anti-barnacle active fluorescence-labeled probe compounds and direct observation of the target region in barnacle cypris larvae for dimethyl-isocyanoalkyl compounds. Tetrahedron ober 17; 61(42): 9969-9973, 2005

Retention of enzyme activity following freeze-drying the mycelium of ectomycorrhizal isolates. II. Enzymes acting upon carbon compounds. World journal of microbiology and biotechnology6(8-9): 865-868, 2000

Retention of enzyme activity following freeze-drying the mycelium of ectomycorrhizal isolates: Part II. Enzymes acting upon carbon compounds. World Journal of Microbiology & Biotechnology 16(8-9): 865-868, 2000

Precision of reversible and irreversible hydrocolloids and of addition-curing silicones used as impression materials in crown and inlay technics. Tandlaegebladet 86(18): 626-631, 1982

Deletion of Braun lipoprotein gene (lpp) and curing of plasmid pPCP1 dramatically alter the virulence of Yersinia pestis CO92 in a mouse model of pneumonic plague. Microbiology 155(Pt 10): 3247-3259, 2009

Studies of the specificity of kinin-forming enzymes the inhibition of the kininogenic activity of trypsin and kallikreins by model compounds. Proceedings of the international symposium on hypotensive peptides, 25-29 October, 1965, Florence, Italy 175-184, 1966

A study of lignin model compounds in phenol-formaldehyde glue reactions. Bi-m. Res. Notes. 27: 3, 23-4, 1971

New curing system of urea-formaldehyde resins with polyhydrazides I. Curing with dihydrazide compounds. Mokuzai Gakkaishi = Journal of the Japan Wood Research Society 35(5): 455-459, 1989

New curing system of urea-formaldehyde resins with polyhydrazides. Curing with dihydrazide compounds. Forest Products Industries 9(1): 162-166, 1990

Porcine, mouse and human galactose 3-O-sulphotransferase-2 enzymes have different substrate specificities; the porcine enzyme requires basic compounds for its catalytic activity. Biochemical Journal 391(Pt 1): 77-85, 2005

Development of aroma-bearing compounds and their precursors in flue-cured tobacco during curing and post-curing operations. Tobacco Research 12(2): 175-185, 1986