+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comprehensive analyses of hydrolyzed wheat protein using shotgun proteomics



Comprehensive analyses of hydrolyzed wheat protein using shotgun proteomics



Kokuritsu Iyakuhin Shokuhin Eisei Kenkyujo Hokoku 2014(131): 50-57



Hydrolyzed wheat protein (HWP; hydrolyzed gluten) is used in various types of products worldwide. Several cases of wheat-dependent, exercise-induced anaphylaxis following exposure to HWP (Glupearl 19S) in cosmetics have been reported. Glupearl 19S was produced from the gluten after partial hydrolysis with hydrogen chloride, and its allergenicity is larger than that of gluten (Adachi R., Allergy 2012;67:1392-9.). It is considered that provocation of allergic manifestations is caused by deamidated gluten in food and/or non-food products. Moreover, an increasing number of studies have shown that HWP can induce IgE-mediated hypersensitivity by skin contact and/or food ingestion. However, the essential molecular properties and profiles of HWP are still unknown. In this study, bioinformatic and multivariate analyses using shotgun proteomics have revealed that 27 proteins significantly decreased in Glupearl 19S compared with intact gluten as shown by the ratio of ion signal intensity of tryptic peptides. In contrast, a single protein significantly increased in HWP compared with intact gluten as shown by the ratio of ion signal intensity of tryptic peptides. Furthermore, we have identified six Glupearl 19S-specific peptides using shotgun proteomics, database searches on Mascot Sequence Query, and de novo sequencing. The six peptides were identified as the specific markers of Glupearl 19S.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 052269658

Download citation: RISBibTeXText

PMID: 24340670


Related references

Comprehensive transcriptomics, proteomics, and metabolomics analyses of the mechanisms regulating tiller production in low-tillering wheat. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 2019, 2019

Bottom-up and shotgun proteomics to identify a comprehensive cochlear proteome. Journal of Visualized Experiments 2014(85), 2014

Safety Assessment of Hydrolyzed Wheat Protein and Hydrolyzed Wheat Gluten as Used in Cosmetics. International Journal of Toxicology 37(1_suppl): 55s-66s, 2018

Temporal Analyses of Barley Malting Stages Using Shotgun Proteomics. Proteomics 18(15): E1800025, 2018

Novel application of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) in shotgun proteomics: comprehensive profiling of rat kidney proteome. Journal of Proteome Research 9(7): 3520-3526, 2010

Application of shotgun proteomics for discovery-driven protein-protein interaction. Methods in Molecular Biology 1156: 265-278, 2014

Protein analysis by shotgun/bottom-up proteomics. Chemical Reviews 113(4): 2343-2394, 2013

Computational approaches to protein inference in shotgun proteomics. Bmc Bioinformatics 13 Suppl 16: S4, 2013

Combining analyses of basophil allergen threshold sensitivity, CD-sens, and IgE antibodies to hydrolyzed wheat, ω-5 gliadin and timothy grass enhances the prediction of wheat challenge outcome. International Archives of Allergy and Immunology 162(1): 50-57, 2013

A bayesian approach to protein inference problem in shotgun proteomics. Journal of Computational Biology 16(8): 1183-1193, 2009

Informatics of protein and posttranslational modification detection via shotgun proteomics. Methods in Molecular Biology 1002: 167-179, 2014

Advancement in protein inference from shotgun proteomics using peptide detectability. Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing 2007: 409-420, 2007

An automated multidimensional protein identification technology for shotgun proteomics. Analytical Chemistry 73(23): 5683-5690, 2002

PAnalyzer: a software tool for protein inference in shotgun proteomics. Bmc Bioinformatics 13: 288, 2013

Spectral index for assessment of differential protein expression in shotgun proteomics. Journal of Proteome Research 7(3): 845-854, 2008