+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comprehensive analysis of elastase-induced pulmonary emphysema in mice: effects of ambient existing particulate matters



Comprehensive analysis of elastase-induced pulmonary emphysema in mice: effects of ambient existing particulate matters



International Immunopharmacology 10(11): 1380-1389



Pulmonary exposure of rodents to porcine pancreatic elastase (PPE) induces lesions that morphologically resemble human panacinar emphysema. However, there has been little work on the comprehensive analysis of this model. The present study was designed to extensively examine the biological effects of PPE on inflammation, cell damage, emphysematous change, and cholinergic reactivity in the lungs of mice. Furthermore, we evaluated the effects of pulmonary exposure to diesel exhaust particles (DEP) on the disease model. Intratracheal administration of PPE induced (1) proinflammatory response in the lungs that was characterized by significant infiltration of leukocytes such as macrophages, eosinophils, and lymphocytes and an increased level of interleukin-1β in lung homogenates, (2) lung cell damage, indicated by higher levels of total protein, lactate dehydrogenase, and alkaline phosphatase in lung homogenates, (3) emphysema-related morphological changes including airspace enlargement and progressive destruction of alveolar wall structures, and (4) airway responsiveness to methacholine in the context of the compliance value of the respiratory system in a dose-dependent manner showing an overall trend. A single intratracheal administration of DEP did not significantly facilitate the hallmark of the disease. This is the first study to extensively analyze PPE-induced lung emphysema in mice with evaluation of the effects of DEP. Furthermore, this bioassay may be applied to future investigations that evaluate new therapeutic agents or risk factors for pulmonary emphysema.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052269764

Download citation: RISBibTeXText

PMID: 20800710

DOI: 10.1016/j.intimp.2010.07.016


Related references

Protective effect of pulmonary surfactant on elastase-induced emphysema in mice. European Respiratory Journal 5(10): 1223-1230, 1992

IL-17A is essential to the development of elastase-induced pulmonary inflammation and emphysema in mice. Respiratory Research 14: 5-5, 2013

Curcumin attenuates elastase- and cigarette smoke-induced pulmonary emphysema in mice. American Journal of Physiology. Lung Cellular and Molecular Physiology 296(4): L614-L623, 2009

Keratinocyte growth factor protects against elastase-induced pulmonary emphysema in mice. American Journal of Physiology. Lung Cellular and Molecular Physiology 293(5): L1230-9, 2007

Adrenomedullin regenerates alveoli and vasculature in elastase-induced pulmonary emphysema in mice. American Journal of Respiratory and Critical Care Medicine 172(5): 581-589, 2005

Perspectives on synergic biological effects induced by ambient allergenic pollen and urban fine/ultrafine particulate matters in atmosphere. Huan Jing Ke Xue= Huanjing Kexue 31(9): 2260-2266, 2011

Annexin V decreases PS-mediated macrophage efferocytosis and deteriorates elastase-induced pulmonary emphysema in mice. American Journal of Physiology. Lung Cellular and Molecular Physiology 303(10): L852-L860, 2013

Reversal of elastase-induced pulmonary emphysema and promotion of alveolar epithelial cell proliferation by simvastatin in mice. American Journal of Physiology. Lung Cellular and Molecular Physiology 294(5): L882-L890, 2008

Inhibition of elastase-induced acute inflammation and pulmonary emphysema in hamsters by a novel neutrophil elastase inhibitor FR901277. Inflammation Research 48(3): 160-167, March, 1999

Studies on the origin of pulmonary emphysema--intrapulmonary elastolytic activities of elastase-induced emphysema. Josai Shika Daigaku Kiyo. Bulletin of the Josai Dental University 7(1): 145-150, 1978

Adenovirus-mediated transfer and overexpression of heme oxygenase 1 cDNA in lungs attenuates elastase-induced pulmonary emphysema in mice. Human Gene Therapy 16(3): 318-327, 2005

Extensive Analysis of Elastase-Induced Pulmonary Emphysema in Rats: ALP in the Lung, a New Biomarker for Disease Progression?. Journal of Clinical Biochemistry and Nutrition 46(2): 168-176, 2010

Effects of airborne fine particulate matters on pulmonary inflammation injury and Th17/Treg balance of sub-chronic exposure mice. Wei Sheng Yan Jiu 43(3): 387-392, 2015

Comparative effects of reversible and irreversible specific elastase inhibitors on elastase-induced emphysema. Bulletin Europeen de Physiopathologie Respiratoire 16 Suppl: 407-413, 1980