+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comprehensive analysis of expressed sequence tags from the pulp of the red mutant 'Cara Cara' navel orange (Citrus sinensis Osbeck)



Comprehensive analysis of expressed sequence tags from the pulp of the red mutant 'Cara Cara' navel orange (Citrus sinensis Osbeck)



Journal of Integrative Plant Biology 52(10): 856-867



Expressed sequence tag (EST) analysis of the pulp of the red-fleshed mutant 'Cara Cara' navel orange provided a starting point for gene discovery and transcriptome survey during citrus fruit maturation. Interpretation of the EST datasets revealed that the mutant pulp transcriptome held a high section of stress responses related genes, such as the type III metallothionein-like gene (6.0%), heat shock protein (2.8%), Cu/Zn superoxide dismutase (0.8%), late embryogenesis abundant protein 5 (0.8%), etc. 133 transcripts were detected to be differentially expressed between the red mutant and its orange-color wild genotype 'Washington' via digital expression analysis. Among them, genes involved in metabolism, defense/stress and signal transduction were statistical overrepresented. Fifteen transcription factors, composed of NAM, ATAF, and CUC transcription factor (NAC); myeloblastosis (MYB); myelocytomatosis (MYC); basic helix-loop-helix (bHLH); basic leucine zipper (bZIP) domain members, were also included. The data reflected the distinct expression profile and the unique regulatory module associated with these two genotypes. Eight differently expressed genes analyzed in digital were validated by quantitative real-time polymerase chain reaction. For structural polymorphism, both simple sequence repeats and single nucleotide polymorphisms (SNP) loci were surveyed; dinucleotide presentation revealed a bias toward AG/GA/TC/CT repeats (52.5%), against GC/CG repeats (0%). SNPs analysis found that transitions (73%) outnumbered transversions (27%). Seventeen potential cultivar-specific and 387 heterozygous SNP loci were detected from 'Cara Cara' and 'Washington' EST pool.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052269774

Download citation: RISBibTeXText

PMID: 20883438

DOI: 10.1111/j.1744-7909.2010.00952.x


Related references

Construction and characterization of a cDNA library from the pulp of Cara Cara navel orange (Citrus sinensis Osbeck). Journal of Integrative Plant Biology 48(3): 315-319, 2006

Effect of salicylic acid on the antioxidant system in the pulp of 'Cara cara' navel orange (Citrus sinensis L. Osbeck) at different storage temperatures. Postharvest Biology and Technology 47(2): 168-175, 2008

Lycopene-epsilon-cyclase pre-mRNA is alternatively spliced in Cara Cara navel orange (Citrus sinensis Osbeck). Biotechnology Letters 27(11): 779-782, 2005

Expression of phytoene synthase gene (Psy) is enhanced during fruit ripening of Cara Cara navel orange (Citrus sinensis Osbeck). Plant Cell Reports 26(6): 837-843, 2007

Changes in dietary fibre, polygalacturonase, cellulase of navel orange (Citrus sinensis (L.) Osbeck 'Cara Cara') fruits under different storage conditions. Scientia Horticulturae 116(4): 0-420, 2008

Cloning and characterization of a novel cDNA encoding late embryogenesis-abundant protein 5 like (LEA-5) gene from Cara Cara navel orange fruit (Citrus sinensis Osbeck). Agricultural Sciences in China 5(4): 272-276, 2006

Carotenoid accumulation in postharvest "Cara Cara" navel orange (Citrus sinensis Osbeck) fruits stored at different temperatures was transcriptionally regulated in a tissue-dependent manner. Plant Cell Reports 31(9): 1667-1676, 2012

Effect of pre-harvest salicylic acid spray treatment on post-harvest antioxidant in the pulp and peel of 'Cara cara' navel orange (Citrus sinenisis L. Osbeck). Journal of the Science of Food and Agriculture 88(2): 229-236, 2008

Daily Consumption of Orange Juice from Citrus sinensis L. Osbeck cv. Cara Cara and cv. Bahia Differently Affects Gut Microbiota Profiling as Unveiled by an Integrated Meta-Omics Approach. Journal of Agricultural and Food Chemistry 67(5): 1381-1391, 2019

Changes in dietary fibre, polygalacturonase, cellulase of navel orange Osbeck Cara Cara fruits under different storage conditions. Scientia Horticulturae6: 4, 414-420, 2008

Effect of Pasteurization on Flavonoids and Carotenoids in Citrus sinensis (L.) Osbeck cv. 'Cara Cara' and 'Bahia' Juices. Journal of Agricultural and Food Chemistry 65(7): 1371-1377, 2017

Phytoene synthase gene cloning from Citrus sinensis Osbeck cv. Cara Cara and its prokaryotic expression. Agricultural Sciences in China 7(2): 148-156, 2008

Phytoene synthase gene cloning from Citrus sinensis Osbeck cv Cara Cara and its prokaryotic expression. Scientia Agricultura Sinica: 10, 3200-3207, 2008

Identification of differentially expressed genes in a spontaneous altered leaf shape mutant of the navel orange [Citrus sinensis (L.) Osbeck. Plant Physiology and Biochemistry 56: 97, 2012

Effects of exogenous ABA and GA3 on sugar concentration in flesh of cara cara navel orange. Ying Yong Sheng Tai Xue Bao 18(11): 2451-2455, 2007