+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comprehensive analysis of inflammatory immune mediators in vitreoretinal diseases



Comprehensive analysis of inflammatory immune mediators in vitreoretinal diseases



Plos one 4(12): E8158



Inflammation affects the formation and the progression of various vitreoretinal diseases. We performed a comprehensive analysis of inflammatory immune mediators in the vitreous fluids from total of 345 patients with diabetic macular edema (DME, n = 92), proliferative diabetic retinopathy (PDR, n = 147), branch retinal vein occlusion (BRVO, n = 30), central retinal vein occlusion (CRVO, n = 13) and rhegmatogenous retinal detachment (RRD, n = 63). As a control, we selected a total of 83 patients with either idiopathic macular hole (MH) or idiopathic epiretinal membrane (ERM) that were free of major pathogenic intraocular changes, such as ischemic retina and proliferative membranes. The concentrations of 20 soluble factors (nine cytokines, six chemokines, and five growth factors) were measured simultaneously by multiplex bead analysis system. Out of 20 soluble factors, three factors: interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant protein-1 (MCP-1) were significantly elevated in all groups of vitreoretinal diseases (DME, PDR, BRVO, CRVO, and RRD) compared with control group. According to the correlation analysis in the individual patient's level, these three factors that were simultaneously increased, did not show any independent upregulation in all the examined diseases. Vascular endothelial growth factor (VEGF) was significantly elevated in patients with PDR and CRVO. In PDR patients, the elevation of VEGF was significantly correlated with the three factors: IL-6, IL-8, and MCP-1, while no significant correlation was observed in CRVO patients. In conclusion, multiplex bead system enabled a comprehensive soluble factor analysis in vitreous fluid derived from variety of patients. Major three factors: IL-6, IL-8, and MCP-1 were strongly correlated with each other indicating a common pathway involved in inflammation process in vitreoretinal diseases.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052269806

Download citation: RISBibTeXText

PMID: 19997642

DOI: 10.1371/journal.pone.0008158


Related references

Comprehensive analysis of inflammatory immune mediators of the intraocular fluid aspirated from the foldable capsular vitreous body filled-eyes. Plos one 7(10): E46384, 2012

Immune mediators in vitreous fluids from patients with vitreoretinal B-cell lymphoma. Investigative Ophthalmology and Visual Science 53(9): 5395-5402, 2012

The role of biomechanics in analysis of cardiovascular diseases Regulation of the fluid shear response by inflammatory mediators. Guilak, Farshid , Reprint Author, Butler, David L, Goldstein, Steven A, Mooney, David J Functional tissue engineering: 337-346, 2003

Morin, a Bioflavonoid Suppresses Monosodium Urate Crystal-Induced Inflammatory Immune Response in RAW 264.7 Macrophages through the Inhibition of Inflammatory Mediators, Intracellular ROS Levels and NF-κB Activation. Plos one 10(12): E0145093, 2015

Inflammatory mediators in chronic inflammatory bowel diseases. Klinische Wochenschrift 69(21-23): 981-987, 1991

Immune complexes and acute phase proteins in chronic inflammatory joint diseases chronic inflammatory bowel diseases parasitic diseases and lymphoma. European Journal of Clinical Investigation 12(2 Part 2): 22, 1982

Effects of interleukin-10 on expression of inflammatory mediators and anti-inflammatory mediators during acute lung injury in rats. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 17(6): 338-341, 2005

Experimental evidence of ω-3 polyunsaturated fatty acid modulation of inflammatory cytokines and bioactive lipid mediators: their potential role in inflammatory, neurodegenerative, and neoplastic diseases. Biomed Research International 2013: 743171, 2013

Lipid mediators of inflammatory and immune reactions. Jpen. Journal of Parenteral and Enteral Nutrition 12(6 Suppl.): 37s-42s, 1988

The chemical and immune mediators of the inflammatory response. Meade, Gordon M (Ed ) Frontiers Of Medicine Symposium Of The 50th Anniversary Of The University Of Rochester Medical Center, Rochester, N Y , Usa, April 1-2, 1976 X+232p Illus Plenum Press: New York, N Y , Usa; London, England ISBN 0-306-31048-1: 105-144, 1977

Role of immune and inflammatory mediators in CNS injury. Drug News and Perspectives 13(3): 133-140, 2000

Astrocytes as mediators of immune and inflammatory responses in the CNS. Neurotoxicology 19(2): 269-281, 1998

Review: Immune cells and mediators of inflammatory arthritis. Autoimmunity 41(3): 224-229, 2008

Inflammatory mediators and immune response in Mexican adolescents. Nutricion Hospitalaria 26(5): 1115-1119, 2012

Analysis of chemical mediators in inflammatory reactions and the mechanism of anti inflammatory drugs. Yakugaku Zasshi 108(4): 251-270, 1988