+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comprehensive analysis of lipid composition in crude palm oil using multiple lipidomic approaches

Comprehensive analysis of lipid composition in crude palm oil using multiple lipidomic approaches

Journal of Genetics and Genomics 41(5): 293-304

Palm oil is currently the leading edible oil consumed worldwide. Triacylglycerol (TAG) and diacylglycerol (DAG) are the dominant lipid classes in palm oil. Other lipid classes present in crude palm oil, such as phospholipids and galactolipids, are very low in abundance. These low-abundance lipids constitute key intermediates in lipid biosynthesis. In this study, we applied multiple lipidomic approaches, including high-sensitivity and high-specificity multiple reaction monitoring, to comprehensively quantify individual lipid species in crude palm oil. We also established a new liquid chromatography-coupled mass spectrometry method that allows direct quantification of low-abundance galactolipids in palm oil without the need for sample pretreatment. As crude palm oil contains large amounts of neutral lipids, our direct-detection method circumvents many of the challenges encountered with conventional lipid quantification methods. This approach allows direct measurement of lipids with no hassle during sample preparation and is more accurate and precise compared with other methods.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 052269813

Download citation: RISBibTeXText

PMID: 24894356

DOI: 10.1016/j.jgg.2014.04.002

Related references

ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis. Methods in Molecular Biology 1691: 125-137, 2017

Lipidomic analysis of the liver from high-fat diet induced obese mice identifies changes in multiple lipid classes. Experimental and Molecular Pathology 97(1): 37-43, 2014

Proteomic and Lipidomic Analysis of Nanoparticle Corona upon Contact with Lung Surfactant Reveals Differences in Protein, but Not Lipid Composition. Acs Nano 9(12): 11872-11885, 2016

Comprehensive Analysis of Changes in Crude Oil Chemical Composition during Biosouring and Treatments. Environmental Science & Technology, 2018

Ethanol production and maximum cell growth are highly correlated with membrane lipid composition during fermentation as determined by lipidomic analysis of 22 Saccharomyces cerevisiae strains. Applied and Environmental Microbiology 79(1): 91-104, 2013

Comprehensive analysis of lipid dynamics variation with lipid composition and hydration of bicelles using nuclear magnetic resonance (NMR) spectroscopy. Langmuir 25(12): 7010-7018, 2009

Comprehensive Metabolomic, Lipidomic and Microscopic Profiling of Yarrowia lipolytica during Lipid Accumulation Identifies Targets for Increased Lipogenesis. Plos One 10(4): E0123188-E0123188, 2016

Lipidomic and proteomic analysis of Caenorhabditis elegans lipid droplets and identification of ACS-4 as a lipid droplet-associated protein. Biochimica et Biophysica Acta 1851(10): 1337-1345, 2015

Lipid changes after leaf wounding in Arabidopsis thaliana: expanded lipidomic data form the basis for lipid co-occurrence analysis. Plant Journal 80(4): 728-743, 2016

A study of some quality aspects of crude palm oil - the stabilisation of crude palm oil at the mill. Palm oil product technology in the eighties: 231-247, 1983

A lipidomic approach to identify cold-induced changes in Arabidopsis membrane lipid composition. Methods in Molecular Biology 1166: 199-215, 2015

Crude palm oil fuel for diesel-engines: Experimental and ANN simulation approaches. Energy 36(8): 4871-4878, 2011

Geometrical isomers of the major provitamin a palm carotenes, alpha - and beta -carotenes in the mesocarp oil of fresh and sterilized palm fruits, crude palm oil and palm carotene-based products: red palm olein and carotene concentrates. Journal of Oil Palm Research 13(2): 23-32, 2001

Chylomicron fatty acid composition and serum lipid concentrations in subjects fed caprenin or palm oil/palm kernel oil as the major dietary fat. Nutrition Research 16(6): 925-936, 1996

Postprandial chylomicron fatty acid composition and serum lipid concentrations in subjects fed caprenin or palm/palm kernel oil as the major dietary fat. FASEB Journal 7(3-4): A170, 1993