+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comprehensive analysis of nonenzymatic post-translational β-lactoglobulin modifications in processed milk by ultrahigh-performance liquid chromatography-tandem mass spectrometry



Comprehensive analysis of nonenzymatic post-translational β-lactoglobulin modifications in processed milk by ultrahigh-performance liquid chromatography-tandem mass spectrometry



Journal of Agricultural and Food Chemistry 61(28): 6971-6981



Nonenzymatic post-translational protein modifications (nePTMs) result in changes of the protein structure that may severely influence physiological and technological protein functions. In the present study, ultrahigh-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) was applied for the systematic identification and site-specific analysis of nePTMs of β-lactoglobulin in processed milk. For this purpose, β-lactoglobulin, which had been heated with lactose under conditions to force nePTM formation (7 d/60 °C), was screened for predicted modifications by using full scans and enhanced resolution scan experiments combined with enhanced product ion scans. Thus, the main glycation, glycoxidation, oxidation, and deamidation products of lysine, arginine, methionine, cysteine, tryptophan, and asparagine, as well as the N-terminus, were identified. Using these MS data, a very sensitive scheduled multiple reaction monitoring method suitable for the analysis of milk products was developed. Consequently, 14 different PTM structures on 25 binding sites of β-lactoglobulin were detected in different milk products.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052269840

Download citation: RISBibTeXText

PMID: 23772976

DOI: 10.1021/jf401549j


Related references

Comprehensive Analysis of Nonenzymatic Post-Translational -Lactoglobulin Modifications in Processed Milk by Ultrahigh-Performance Liquid Chromatographya Tandem Mass Spectrometry. 2013

Simultaneous Determination of Naturally Occurring Estrogens and Mycoestrogens in Milk by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry Analysis. Journal of Agricultural and Food Chemistry 63(40): 8940-8946, 2016

Quantitative analysis of gangliosides in bovine milk and colostrum-based dairy products by ultrahigh performance liquid chromatography-tandem mass spectrometry. Journal of Agricultural and Food Chemistry 61(40): 9689-9696, 2014

Quality analysis of Polygala tenuifolia root by ultrahigh performance liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry. Journal of Food and Drug Analysis 23(1): 144-151, 2015

ProteomeTools: Systematic Characterization of 21 Post-translational Protein Modifications by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) Using Synthetic Peptides. Molecular and Cellular Proteomics 17(9): 1850-1863, 2018

Rapid analysis of aflatoxin M1 in milk using dispersive liquid-liquid microextraction coupled with ultrahigh pressure liquid chromatography tandem mass spectrometry. Analytical and Bioanalytical Chemistry 405(26): 8645-8652, 2014

Quantification of ampicillin in bovine milk by coupled-column ultrahigh-performance liquid chromatography-tandem mass spectrometry. Journal of Separation Science 35(19): 2615-2620, 2013

Analysis of 18 perfluorinated compounds in river waters: comparison of high performance liquid chromatography-tandem mass spectrometry, ultra-high-performance liquid chromatography-tandem mass spectrometry and capillary liquid chromatography-mass spectrometry. Journal of Chromatography. a 1244: 88-97, 2012

Analysis of non-derivatised bacteriohopanepolyols by ultrahigh-performance liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry 30(19): 2087-2098, 2018

Peptide sequencing and characterization of post-translational modifications by enhanced ion-charging and liquid chromatography electron-transfer dissociation tandem mass spectrometry. Analytical Chemistry 79(24): 9243-9252, 2007

Determination of Nine Bisphenol-Diglycidyl Ethers in Human Breast Milk by Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry. Journal of Agricultural and Food Chemistry 66(37): 9810-9818, 2018

Nonesterified fatty acid determination for functional lipidomics: comprehensive ultrahigh performance liquid chromatography-tandem mass spectrometry quantitation, qualification, and parameter prediction. Analytical Chemistry 84(3): 1483-1490, 2012

Phytochemical analyses of Ziziphus jujuba Mill. var. spinosa seed by ultrahigh performance liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry. Analyst 138(22): 6881-6888, 2014

Ultra-high performance liquid chromatography-mass spectrometry for the fast profiling of histone post-translational modifications. Journal of Proteome Research 9(10): 5501-5509, 2010

Towards liquid chromatography time-scale peptide sequencing and characterization of post-translational modifications in the negative-ion mode using electron detachment dissociation tandem mass spectrometry. Journal of the American Society for Mass Spectrometry 19(8): 1156-1162, 2008