+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comprehensive analysis of phosphorylated proteins of Escherichia coli ribosomes



Comprehensive analysis of phosphorylated proteins of Escherichia coli ribosomes



Journal of Proteome Research 8(7): 3390-3402



Phosphorylation of bacterial ribosomal proteins has been known for decades; however, there is still very limited information available on specific locations of the phosphorylation sites in ribosomal proteins and the role they might play in protein synthesis. In this study, we have mapped the specific phosphorylation sites in 24 Escherichia coli ribosomal proteins by tandem mass spectrometry. Detection of phosphorylation was achieved by either phosphorylation specific visualization techniques, ProQ staining, and antibodies for phospho-Ser, Thr, and Tyr; or by mass spectrometry equipped with a capability to detect addition and loss of the phosphate moiety. Enrichment by immobilized metal affinity and/or strong cation exchange chromatography was used to improve the success of detection of the low abundance phosphopeptides. We found the small subunit (30S) proteins S3, S4, S5, S7, S11, S12, S13, S18, and S21 and the large subunit (50S) proteins L1, L2, L3, L5, L6, L7/L12, L13, L14, L16, L18, L19, L21, L22, L28, and L31 to be phosphorylated at one or more residues. Potential roles for each specific site in ribosome function were deduced through careful evaluation of the given phosphorylation sites in 3D-crystal structure models of ribosomes and the previous mutational studies of E. coli ribosomal proteins.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052269850

Download citation: RISBibTeXText

PMID: 19469554

DOI: 10.1021/pr900042e


Related references

Properties of ribosomes and RNA synthesized by Escherichia coli grown in the presence of ethionine. 3. Methylated proteins in 50 S ribosomes of E. coli EA2. Journal of Molecular Biology 86(1): 139-159, 1974

Immunological analysis of the proteins of Escherichia coli ribosomes. Journal of Molecular Biology 20(3): 447-452, 1966

Acid proteins of eukaryotic cell ribosomes isolation of a protein similar to escherichia coli l 7 l 12 from 80s pea ribosomes. Biokhimiya 47(11): 1899-1906, 1982

Analysis of kethoxal bound to ribosomal proteins from Escherichia coli 70S reacted ribosomes. Biochemical and Biophysical Research Communications 60(3): 901-908, 1974

Analysis of the ribosomes engaged in the synthesis of the outer membrane proteins of Escherichia coli. Molecular and General Genetics 137(2): 151-160, 1975

Structure and function of Escherichia coli ribosomes. 3. Stoichiometry and rate of the reconstitution of ribosomes from subribosomal particles and split proteins. Journal of Molecular Biology 34(3): 609-619, 1968

Electrophoretic analysis of proteins from normal and cesium chloride-treated Escherichia coli ribosomes. Journal of Molecular Biology 24(2): 149-155, 1967

Interaction of escherichia coli ribosomes with chloramphenicol the effect of basic proteins on some properties of ribosomes and their capacity for chloramphenicol binding. Antibiotiki 19(3): 216-219, 1974

Studies on proteins of animal ribosomes. XIV. Analysis of phosphorylated rat liver ribosomal proteins by two-dimensional polyacrylamide gel electrophoresis. Febs Letters 26(1): 233-236, 1972

A study of the mechanism of translocation in ribosomes. III. Influence of the alkylation of the SH groups of the proteins of the ribosomal 30S subparticle on "nonenzymatic" translocation in the ribosomes of Escherichia coli. Molecular Biology 8(3): 362-366, 1974

The mechanism of translocation in ribosomes part 3 effect of alkylation of sulfhydryl groups of the ribosomal 30s sub particle proteins on the nonenzymatic translocation in escherichia coli ribosomes. Molekulyarnaya Biologiya 8(3): 452-457, 1974

Ribosomal proteins. XI. Isolation of proteins form 70S ribosomes of Escherichia coli. Hoppe-Seyler's Zeitschrift für Physiologische Chemie 352(11): 1545-1552, 1971

Ribosomal proteins part 11 isolation of proteins from 70s ribosomes of escherichia coli. Hoppe Seyler'S Zeitschrift Fuer Physiologische Chemie1: 1545-1552, 1971

Ribosomal proteins. XXXII. Comparison of several extraction methods for proteins from Escherichia coli ribosomes. Biochimie 54(2): 167-175, 1972

50-S ribosomal proteins. Peptide studies on two acidic proteins, A 1 and A 2 , isolated from 50-S ribosomes of Escherichia coli. European Journal of Biochemistry 25(1): 13-19, 1972