+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comprehensive analysis of the clinical significance of inducing pluripotent stemness-related gene expression in colorectal cancer cells



Comprehensive analysis of the clinical significance of inducing pluripotent stemness-related gene expression in colorectal cancer cells



Annals of Surgical Oncology 16(9): 2638-2644



We previously determined that cancer stem-like cells may influence the susceptibility of colorectal cancer (CRC) cells to chemotherapeutic agents. Although Takahashi and Park identified a set of induced pluripotent stem cell (iPS)-related genes required for normal stem cell maintenance, the precise role of iPS-related gene expression in CRC pathogenesis remains to be determined. The purpose of this study was to clarify the clinical relevance of "stemness"-regulating gene expression in CRC cases. Cancer cells were excised from tissues of 79 CRC cases by laser microdissection (LMD), and quantitative RT-PCR was used to evaluate expression levels of the iPS-related genes c-MYC, SOX2, OCT3/4, LIN28, KLF4, and NANOG, and to identify any associations between their expression and clinicopathological CRC progression. We found that LIN28 expression is significantly associated with lymph node metastasis (p = 0.018) and Dukes stage (p = 0.0319). SOX2expression is also correlated with lymph node metastasis. Furthermore, the ten cases with Dukes D disease expressed significantly higher levels of SOX2transcript than the other 69 cases (p = 0.0136). In contrast, KLF4 expression was inversely related to Dukes stage. Expression of c-MYC, OCT3/4, and NANOG did not appear to have clinical relevance in CRC cases. The present analysis strongly suggests that altered expression of several iPS-related genes plays a role in CRC pathogenesis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052269891

Download citation: RISBibTeXText

PMID: 19554373

DOI: 10.1245/s10434-009-0567-5


Related references

Clinicopathological significance of the gene expression of matrix metalloproteinases and reversion-inducing cysteine-rich protein with Kazal motifs in patients with colorectal cancer: MMP-2 gene expression is a useful predictor of liver metastasis from colorectal cancer. Oncology Reports 19(5): 1285-1291, 2008

Comprehensive expression analysis of TNF-related apoptosis-inducing ligand and its receptors in colorectal cancer: Correlation with MAPK alterations and clinicopathological associations. Pathology, Research and Practice, 2018

Statins augment the chemosensitivity of colorectal cancer cells inducing epigenetic reprogramming and reducing colorectal cancer cell 'stemness' via the bone morphogenetic protein pathway. Gut 60(11): 1544-1553, 2012

Global gene expression profiling analysis reveals reduction of stemness after B-RAF inhibition in colorectal cancer cell lines. Genomics Data 4: 158-161, 2015

The expression of mutation p53 gene from circulating cancer cells of peripheral blood and the clinical significance in detecting the patients with colorectal cancer. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 23(2): 186-188, 2006

The expression and clinical significance of β-catenin and colorectal cancer stem cells marker EpCAMhigh/CD44+in colorectal cancer. Chinese-German Journal of Clinical Oncology 12(12): 581-585, 2013

Clinical significance of human kallikrein 10 gene expression in colorectal cancer and gastric cancer. Journal of Gastroenterology and Hepatology 21(10): 1596-1603, 2006

Expression and clinical significance of metastasis-related tumor markers in colorectal cancer. Ai Zheng 28(9): 950-954, 2011

Clinical significance of human kallikrein7 gene expression in colorectal cancer. Annals of Surgical Oncology 17(11): 3037-3042, 2011

Expression and clinical significance of ATM and PUMA gene in patients with colorectal cancer. Oncology Letters 14(6): 7825-7828, 2018

Clinical significance of STC1 gene expression in patients with colorectal cancer. Anticancer Research 31(1): 325-329, 2011

Clinical significance of kallikrein-related peptidase (KLK10) mRNA expression in colorectal cancer. Clinical Biochemistry 46(15): 1453-1461, 2014

Expression and clinical significance of hedgehog signaling pathway related components in colorectal cancer. Asian Pacific Journal of Cancer Prevention 13(5): 2319-2324, 2013

Clinical significance of mismatch repair gene expression in sporadic colorectal cancer. Experimental and Therapeutic Medicine 8(5): 1416-1422, 2014

Clinical significance of human kallikrein gene 6 messenger RNA expression in colorectal cancer. Clinical Cancer Research 11(8): 2889-2893, 2005