+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comprehensive analysis of tissue preservation and recording quality from chronic multielectrode implants



Comprehensive analysis of tissue preservation and recording quality from chronic multielectrode implants



Plos One 6(11): E27554



Multielectrodes have been used with great success to simultaneously record the activity of neuronal populations in awake, behaving animals. In particular, there is great promise in the use of this technique to allow the control of neuroprosthetic devices by human patients. However, it is crucial to fully characterize the tissue response to the chronic implants in animal models ahead of the initiation of human clinical trials. Here we evaluated the effects of unilateral multielectrode implants on the motor cortex of rats weekly recorded for 1-6 months using several histological methods to assess metabolic markers, inflammatory response, immediate-early gene (IEG) expression, cytoskeletal integrity and apoptotic profiles. We also investigated the correlations between each of these features and firing rates, to estimate the impact of post-implant time on neuronal recordings. Overall, limited neuronal loss and glial activation were observed on the implanted sites. Reactivity to enzymatic metabolic markers and IEG expression were not significantly different between implanted and non-implanted hemispheres. Multielectrode recordings remained viable for up to 6 months after implantation, and firing rates correlated well to the histochemical and immunohistochemical markers. Altogether, our results indicate that chronic tungsten multielectrode implants do not substantially alter the histological and functional integrity of target sites in the cerebral cortex.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052269924

Download citation: RISBibTeXText

PMID: 22096594

DOI: 10.1371/journal.pone.0027554


Related references

A new multielectrode for chronic recording of intracortical field potentials in cats. Physiology and Behavior 29(3): 567-571, 1982

Distribution and Morphology of Calcium-Binding Proteins Immunoreactive Neurons following Chronic Tungsten Multielectrode Implants. Plos One 10(6): E0130354, 2016

CMOS image sensor integrated with micro-LED and multielectrode arrays for the patterned photostimulation and multichannel recording of neuronal tissue. Optics Express 20(6): 6097-6108, 2012

High-density multielectrode array with independently maneuverable electrodes and silicone oil fluid isolation system for chronic recording from macaque monkey. Journal of Neuroscience Methods 211(1): 114-124, 2013

Effects of caspase-1 knockout on chronic neural recording quality and longevity: insight into cellular and molecular mechanisms of the reactive tissue response. Biomaterials 35(36): 9620-9634, 2015

Quality control of RNA preservation and extraction from paraffin-embedded tissue: implications for RT-PCR and microarray analysis. Plos One 8(7): E70714, 2014

Multielectrode recording from the vertebrate retina. Investigative Ophthalmology & Visual Science 30(3 SUPPL): 68, 1989

A wire multielectrode for intramuscular recording. Medical and Biological Engineering 12(5): 721-723, 1974

Multielectrode recording in the vomeronasal organ. Society for Neuroscience Abstracts 24(1-2): 909, 1998

A micromachined silicon multielectrode for multiunit recording. Journal of Neuroscience Methods 126(2): 119-126, 30 June, 2003

A new planar multielectrode array: recording from a rat auditory cortex. Journal of Neural Engineering 3(4): 293-298, 2006

A new non-parametric modeling method for multielectrode recording data. Society for Neuroscience Abstracts 23(1-2): 2104, 1997

Silicone gel breast implants and connective tissue disease--a comprehensive review. Autoimmunity 36(4): 193-197, 2003

Preservation and regeneration of alveolar bone by tissue-engineered implants. Tissue Engineering 11(5-6): 751-767, 2005

Comprehensive characterization of tungsten microwires in chronic neurocortical implants. Conference Proceedings 2012: 755-758, 2013