EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Conditional knockout of fibronectin abrogates mouse mammary gland lobuloalveolar differentiation



Conditional knockout of fibronectin abrogates mouse mammary gland lobuloalveolar differentiation



Developmental Biology 346(1): 11-24



Fibronectin (Fn) plays an important part in the branching morphogenesis of salivary gland, lung, and kidney. Here, we examine the effect of the conditional knockout of Fn in the mammary epithelium [Fn(MEp-/-)] on postnatal mammary gland development, using Cre-loxP-mediated gene knockout technology. Our data show that Fn deletion causes a moderate retardation in outgrowth and branching of the ductal tree in 5-week-old mice. These defects are partially compensated in virgin 16-week-old mice. However, mammary glands consisting of Fn-deficient epithelial cells fail to undergo normal lobuloalveolar differentiation during pregnancy. The severity of lobuloalveolar impairment ranged from lobular hypoplasia to aplasia in some cases and was associated with the amount of Fn protein recovered from these glands. Decreased rates of mammary epithelial cell proliferation accounted for delayed ductal outgrowth in virgin and lack of alveologenesis in pregnant Fn(MEp-/-) mice. Concomitant decreased expression of integrin beta(1) (Itgb1) and lack of autophosphorylation of focal adhesion kinase (Fak) suggest that this pathology might, at least in part, be mediated by disruption of the Fn/Itgb1/Fak signaling pathway.

(PDF emailed within 0-6 h: $19.90)

Accession: 052287768

Download citation: RISBibTeXText

PMID: 20624380

DOI: 10.1016/j.ydbio.2010.07.001



Related references

C/EBPb, but not C/EBPa, is essential for ductal morphogenesis, lobuloalveolar proliferation, and functional differentiation in the mouse mammary gland. Genes and Development 12(12): 17-28, 1998

C/EBPbeta, but not C/EBPalpha, is essential for ductal morphogenesis, lobuloalveolar proliferation, and functional differentiation in the mouse mammary gland. Genes & Development 12(12): 1917-1928, 1998

C/EBPbeta , but not C/EBPalpha , is essential for ductal morphogenesis, lobuloalveolar proliferation, and functional differentiation in the mouse mammary gland. Genes & Development 12(12): 1917-1928, 1998

Lobuloalveolar Differentiation In Mouse Mammary Tissues In Vitro. Science 145(3631): 496-497, 1964

Elimination of plasma fibronectin in the adult mouse by a conditional fibronectin knockout Impaired healing of brain tissue. Molecular Biology of the Cell 11(Supplement): 429a, 2000

Lobuloalveolar differentiation and tumorigenesis: two separate activities of mouse mammary tumor virus. Cancer Research 43(12 Pt 1): 5879-5882, 1983

Local effects of EGF, alpha-TGF, and EGF-like growth factors on lobuloalveolar development of the mouse mammary gland in vivo. Journal of Cellular Physiology 132(3): 581-584, 1987

Structure-activity relationship of retinoids on lobuloalveolar differentiation of cultured mouse mammary glands. Carcinogenesis 7(7): 1175-1182, 1986

The breast proto-oncogene, HRGalpha regulates epithelial proliferation and lobuloalveolar development in the mouse mammary gland. Oncogene 21(32): 4900-4907, 2002

Effect of conditional knockout of the type II TGF-beta receptor gene in mammary epithelia on mammary gland development and polyomavirus middle T antigen induced tumor formation and metastasis. Cancer Research 65(6): 2296-2302, 2005

Effect of exogenous epidermal-like growth factors on mammary gland development and differentiation in the estrogen receptor-alpha knockout (ERKO) mouse. Breast Cancer Research and Treatment 79(2): 161-173, 2003

Suppression of epithelial apoptosis and delayed mammary gland involution in mice with a conditional knockout of Stat3. Genes & Development 13(19): 2604-2616, 1999

Suppressed epithelial apoptosis and delayed mammary gland involution in mice with a conditional knockout of Stat3. British Journal of Cancer 80(SUPPL 2): 10, July, 1999

Histamine synthesis by mouse mammary gland epithelial cells in primary culture. The effects of mammary gland differentiation stage (pregnancy and lactation). Inflammation Research 50(Supplement 2): S104-S105, 2001

Cx26 knockout predisposes the mammary gland to primary mammary tumors in a DMBA-induced mouse model of breast cancer. Oncotarget 6(35): 37185-37199, 2016