EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Construction and properties of a herpes simplex virus 2 dl5-29 vaccine candidate strain encoding an HSV-1 virion host shutoff protein


Construction and properties of a herpes simplex virus 2 dl5-29 vaccine candidate strain encoding an HSV-1 virion host shutoff protein



Vaccine 28(15): 2754-2762



ISSN/ISBN: 0264-410X

PMID: 20117270

DOI: 10.1016/j.vaccine.2010.01.030

The replication-defective herpes simplex virus 2 (HSV-2) dl5-29 mutant virus strain with deletions in the U(L)5 and U(L)29 genes has been shown to protect mice and guinea pigs against challenge with wild-type (wt) HSV-2 and to protect against ocular disease caused by HSV-1 infection. The dl5-29 strain is currently being prepared for clinical trials as a herpes vaccine candidate. As a possible approach to improve the efficacy of dl5-29 as a genital herpes vaccine, we replaced the U(L)41 gene encoding the virion host shutoff function (vhs) with the U(L)41 gene from HSV-1. While the HSV-2 U(L)41 and HSV-1 U(L)41 gene products have analogous functions, vhs-1 is 40-fold less active than vhs-2. Previously, it was shown that disruption of the U(L)41 gene can increase the efficacy of dl5-29 as a vaccine against HSV-2. These properties led us to hypothesize that replacement of vhs-2 by vhs-1 would decrease cytopathic effects in infected host cells, allowing longer survival of antigen-presenting cells and induction of stronger immune responses. The new recombinant dl5-29-41.1 virus shows nearly the same immunogenicity and protection against HSV-2 challenge as the parental dl5-29 virus or a triply deleted mutant virus, dl5-29-41, in the murine model of infection, and grows to higher titers than the parental strain in complementing cells, which is important for GMP production. The results have implications for the design of future HSV-2 vaccine candidates and mechanisms of induction of protective immunity against genital herpes.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052311928

Download citation: RISBibTeXText

Related references

Disruption of virion host shutoff activity improves the immunogenicity and protective capacity of a replication-incompetent herpes simplex virus type 1 vaccine strain. Journal of Virology 74(23): 11137-11144, 2000

Differentiation of the shutoff of protein synthesis by virion host shutoff and mutant c134.5 genes of herpes simplex virus 1. Virology 229: 105, 1997

Differentiation of the shutoff of protein synthesis by virion host shutoff and mutant gamma (1)34.5 genes of herpes simplex virus 1. Virology 229(1): 98-105, 1997

Virion component of herpes simplex virus type 1 KOS interferes with early shutoff of host protein synthesis induced by herpes simplex virus type 2 186. Journal of Virology 56(1): 312-316, 1985

Herpes simplex virus type 1 (HSV-1) strain HSZP host shutoff gene: Nucleotide sequence and comparison with HSV-1 strains differing in early shutoff of host protein synthesis. Virus Genes 15(2): 155-159, 1997

Herpes simplex virus VP16 forms a complex with the virion host shutoff protein vhs. Journal of Virology 68(4): 2339-2346, 1994

Herpes simplex virus 1 counteracts viperin via its virion host shutoff protein UL41. Journal of Virology 88(20): 12163-12166, 2014

Production of antisera to the virion host shutoff protein of herpes simplex virus type 1. Abstracts of the General Meeting of the American Society for Microbiology 91: 329, 1991

Evidence for translational regulation by the herpes simplex virus virion host shutoff protein. Journal of Virology 84(12): 6041-6049, 2010

Inhibition of human immunodeficiency virus replication by the herpes simplex virus virion host shutoff protein. Journal of Virology 71(7): 5521-5527, 1997

Herpes simplex virus type 1 abrogates the antiviral activity of Ch25h via its virion host shutoff protein. Antiviral Research 143: 69-73, 2017

Herpes simplex virus virion host shutoff protein: immune evasion mediated by a viral RNase?. Journal of Virology 78(3): 1063-1068, 2004

The virion host shutoff protein of herpes simplex virus type 1: messenger ribonucleolytic activity in vitro. Journal of Virology 70(4): 2411-2419, 1996

Herpes simplex virus 2 virion host shutoff protein interferes with type I interferon production and responsiveness. Virology 322(1): 158-167, 2004

Infected cell protein 22 of herpes simplex virus 1 regulates the expression of virion host shutoff gene U(L)41. Virology 234(2): 226-234, 1997