Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21

Maidin, M.S.T.; Song, A.A.-L.; Jalilsood, T.; Sieo, C.C.; Yusoff, K.; Rahim, R.A.

Plasmid 74: 32-38


ISSN/ISBN: 1095-9890
PMID: 24879963
DOI: 10.1016/j.plasmid.2014.05.003
Accession: 052312615

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A vector that drives the expression of the reporter gusA gene in both Lactobacillus plantarum and Lactococcus lactis was constructed in this study. This vector contained a newly characterized heat shock promoter (Phsp), amplified from an Enterococcus faecium plasmid, pAR6. Functionality and characterization of this promoter was initially performed by cloning Phsp into pNZ8008, a commercial lactococcal plasmid used for screening of putative promoters which utilizes gusA as a reporter. It was observed that Phsp was induced under heat, salinity and alkaline stresses or a combination of all three stresses. The newly characterized Phsp promoter was then used to construct a novel Lactobacillus vector, pAR1801 and its ability to express the gusA under stress-induced conditions was reproducible in both Lb. plantarum Pa21 and L. lactis M4 hosts.