+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Coordinated allele-specific histone acetylation at the differentially methylated regions of imprinted genes

Coordinated allele-specific histone acetylation at the differentially methylated regions of imprinted genes

Nucleic Acids Research 38(22): 7974-7990

Genomic imprinting is an epigenetic inheritance system characterized by parental allele-specific gene expression. Allele-specific DNA methylation and chromatin composition are two epigenetic modification systems that control imprinted gene expression. To get a general assessment of histone lysine acetylation at imprinted genes we measured allele-specific acetylation of a wide range of lysine residues, H3K4, H3K18, H3K27, H3K36, H3K79, H3K64, H4K5, H4K8, H4K12, H2AK5, H2BK12, H2BK16 and H2BK46 at 11 differentially methylated regions (DMRs) in reciprocal mouse crosses using multiplex chromatin immunoprecipitation SNuPE assays. Histone acetylation marks generally distinguished the methylation-free alleles from methylated alleles at DMRs in mouse embryo fibroblasts and embryos. Acetylated lysines that are typically found at transcription start sites exhibited stronger allelic bias than acetylated histone residues in general. Maternally methylated DMRs, that usually overlap with promoters exhibited higher levels of acetylation and a 10% stronger allele-specific bias than paternally methylated DMRs that reside in intergenic regions. Along the H19/Igf2 imprinted domain, allele-specific acetylation at each lysine residue depended on functional CTCF binding sites in the imprinting control region. Our results suggest that many different histone acetyltransferase and histone deacetylase enzymes must act in concert in setting up and maintaining reciprocal parental allelic histone acetylation at DMRs.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 052342547

Download citation: RISBibTeXText

PMID: 20693536

DOI: 10.1093/nar/gkq680

Related references

Interaction between differentially methylated regions partitions the imprinted genes Igf2 and H19 into parent-specific chromatin loops. Nature Genetics 36(8): 889-893, 883, August, 2004

Genome-wide and parental allele-specific analysis of CTCF and cohesin DNA binding in mouse brain reveals a tissue-specific binding pattern and an association with imprinted differentially methylated regions. Genome Research 23(10): 1624-1635, 2014

Allele-specific histone lysine methylation marks regulatory regions at imprinted mouse genes. EMBO (European Molecular Biology Organization) Journal 21(23): 6560-6570, December 2, 2002

A novel approach identifies new differentially methylated regions (DMRs) associated with imprinted genes. Genome Research 21(3): 465-476, 2011

Differentially methylated regions of imprinted genes in prenatal, perinatal and postnatal human tissues. Plos One 7(7): E40924-E40924, 2013

DNA methylation at differentially methylated regions of imprinted genes is resistant to developmental programming by maternal nutrition. Epigenetics 7(10): 1200-1210, 2013

Specific differentially methylated domain sequences direct the maintenance of methylation at imprinted genes. Molecular and Cellular Biology 26(22): 8347-8356, 2006

The use of methylation-sensitive representational difference analysis to identify novel differentially methylated regions/imprinted genes on mouse proximal chromosome 11. Genetical Research 81(3): 235, June, 2003

Characterization of differentially methylated regions in 3 bovine imprinted genes: a model for studying human germ-cell and embryo development. Cytogenetic and Genome Research 132(4): 239-247, 2011

Genome-wide DNA methylation analysis of patients with imprinting disorders identifies differentially methylated regions associated with novel candidate imprinted genes. Journal of Medical Genetics 51(4): 229-238, 2014

Dynamic stage-specific changes in imprinted differentially methylated regions during early mammalian development and prevalence of non-CpG methylation in oocytes. Development 138(5): 811-820, 2011

Bisulfite sequencing and dinucleotide content analysis of 15 imprinted mouse differentially methylated regions (DMRs): paternally methylated DMRs contain less CpGs than maternally methylated DMRs. Cytogenetic and Genome Research 113(1-4): 130-137, 2006

Parentally imprinted allele (PIA) typing in the differentially methylated region upstream of the human H19 gene. Forensic Science International. Genetics 2(4): 286-291, 2009

Maintaining memory of silencing at imprinted differentially methylated regions. Cellular and Molecular Life Sciences 73(9): 1871-1879, 2016

Shared role for differentially methylated domains of imprinted genes. Molecular and Cellular Biology 22(7): 2089-2098, 2002