+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Crystallization and preliminary X-ray crystallographic studies of transglutaminase 2 in complex with Ca2+



Crystallization and preliminary X-ray crystallographic studies of transglutaminase 2 in complex with Ca2+



Acta Crystallographica. Section F Structural Biology Communications 70(Pt 4): 513-516



Transglutaminase 2 (TG2) is a multi-functional protein that has been implicated in a variety of physiological cellular activities, including apoptosis, angiogenesis and cellular differentiation. Two functions of TG2 are protein cross-linking and GTP hydrolysis activities. The protein cross-linking activity of TG2 is positively controlled by calcium; however, the molecular mechanism of its Ca(2+)-dependent activity is completely unknown. In the present study, full-length human TG2 in complex with Ca(2+) was overexpressed, purified and crystallized at 20°C as a first step towards elucidating this mechanism. X-ray diffraction data were collected to a resolution of 3.4 Å from a crystal belonging to space group C2221, with unit-cell parameters a = 133.08, b = 216.30, c = 166.26 Å. Based on these data, the asymmetric unit was estimated to contain three molecules.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052398999

Download citation: RISBibTeXText

PMID: 24699752

DOI: 10.1107/s2053230x1400510x


Related references

Complex assembly, crystallization and preliminary X-ray crystallographic studies of the swine major histocompatibility complex molecule SLA-1*1502. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 5): 568-571, 2011

Crystallization and preliminary X-ray crystallographic studies of the Z-DNA-binding domain of a PKR-like kinase (PKZ) in complex with Z-DNA. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 65(Pt 3): 267-270, 2009

Crystallization and preliminary crystallographic studies of human RIG-I in complex with double-stranded RNA. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 65(Pt 6): 648-650, 2009

Crystallization and preliminary crystallographic analysis of the catalytic domain of human flap endonuclease 1 in complex with a nicked DNA product: use of a DPCS kit for efficient protein-DNA complex crystallization. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 64(Pt 1): 39-43, 2008

Crystallization and preliminary X-ray crystallographic studies of bovine heart mitochondrial cytochrome bc1 complex. Journal of Molecular Biology 221(2): 379-382, 1991

Complex assembly, crystallization and preliminary X-ray crystallographic studies of MHC H-2Kd complexed with an HBV-core nonapeptide. Acta Crystallographica. Section D Biological Crystallography 60(Pt 8): 1473-1475, 2004

Crystallization and preliminary crystallographic studies of RhoGDI in complex with the radixin FERM domain. Acta Crystallographica Section D Biological Crystallography 57(6): 889-890, 2001

Crystallization and preliminary X-ray crystallographic studies of the oxidative-stress sensor SoxR and its complex with DNA. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 62(Pt 12): 1275-1277, 2006

Purification, crystallization and preliminary crystallographic studies of SPCI-chymotrypsin complex at 2.8 A resolution. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 64(Pt 10): 914-917, 2008

Crystallization and preliminary crystallographic studies of CCM3 in complex with the C-terminal domain of MST4. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 68(Pt 7): 760-763, 2012

Crystallization and preliminary crystallographic studies of the HA3 subcomponent of the type B botulinum neurotoxin complex. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 10): 1244-1246, 2011

Purification, crystallization and preliminary crystallographic studies of the complex of interferon-lambda1 with its receptor. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 66(Pt 1): 61-63, 2010

Complex assembly, crystallization and preliminary X-ray crystallographic studies of duck MHC class I molecule. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 66(Pt 1): 99, 2010

Crystallization and preliminary crystallographic studies of the YafN-YafO complex from Escherichia coli. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 68(Pt 8): 894-897, 2012

Purification, crystallization and preliminary X-ray crystallographic studies of the complex between Smc5 and the SUMO E3 ligase Mms21. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 65(Pt 8): 849-852, 2009