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Crystallization and preliminary crystallographic analysis of human LR11 Vps10p domain



Crystallization and preliminary crystallographic analysis of human LR11 Vps10p domain



Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 1): 129-132



Low-density lipoprotein receptor (LDLR) relative with 11 binding repeats (LR11; also known as sorLA) is genetically associated with late-onset Alzheimer's disease and is thought to be involved in neurodegenerative processes. LR11 contains a vacuolar protein-sorting 10 protein (Vps10p) domain. As this domain has been implicated in protein-protein interaction in other receptors, its structure and function are of great biological interest. Human LR11 Vps10p domain was expressed in mammalian cells and the purified protein was crystallized using the hanging-drop vapour-diffusion method. Enzymatic deglycosylation of the sample was critical to obtaining diffraction-quality crystals. Deglycosylated LR11 Vps10p-domain crystals belonged to the hexagonal space group P6(1)22. A diffraction data set was collected to 2.4 Å resolution and a clear molecular-replacement solution was obtained.

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Accession: 052399282

Download citation: RISBibTeXText

PMID: 21206043

DOI: 10.1107/s1744309110048153


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