+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Crystallization and preliminary crystallographic studies of the YafN-YafO complex from Escherichia coli



Crystallization and preliminary crystallographic studies of the YafN-YafO complex from Escherichia coli



Acta Crystallographica. Section F Structural Biology and Crystallization Communications 68(Pt 8): 894-897



The ribosome-dependent mRNA interferase YafO from Escherichia coli belongs to a type II toxin-antitoxin (TA) system and its cognate antitoxin YafN neutralizes cell toxicity by forming a stable YafN-YafO complex. The YafN-YafO TA system is upregulated by the SOS response (a global response to DNA damage in which the cell cycle is arrested and mutagenesis is induced) and may then inhibit protein synthesis by endoribonuclease activity of YafO with the 50S ribosome subunit. Structural information on the YafN-YafO complex and related complexes would be helpful in order to understand the structural basis of the mechanism of mRNA recognition and cleavage, and the assembly of these complexes. Here, the YafN-YafO complex was expressed and crystallized. Crystals grown by the hanging-drop vapour-diffusion method diffracted to 3.50 Å resolution and belonged to the hexagonal space group P622, with unit-cell parameters a = 86.14, b = 86.14, c = 173.11 Å, α = β = 90, γ = 120°. Both Matthews coefficient analysis and the self-rotation function suggested the presence of one molecule per asymmetric unit in the crystal, with a solvent content of 65.69% (V(M) = 3.58 Å(3) Da(-1)).

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052399352

Download citation: RISBibTeXText

PMID: 22869116

DOI: 10.1107/s1744309112024037


Related references

Crystallization and preliminary X-ray crystallographic analysis of FlhD/FlhC complex from Escherichia coli. Acta Crystallographica Section D Biological Crystallography 57(5): 734-736, 2001

Crystallization and preliminary crystallographic analysis of the transcriptional regulator RfaH from Escherichia coli and its complex with ops DNA. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 62(Pt 10): 1027-1030, 2006

Crystallization and preliminary X-ray crystallographic studies of the alkanesulfonate FMN reductase from Escherichia coli. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 61(Pt 9): 837-840, 2005

Crystallization and preliminary crystallographic studies of UbiG, an O-methyltransferase from Escherichia coli. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 6): 727-729, 2011

Crystallization and preliminary X-ray crystallographic studies of Escherichia coli xanthine phosphoribosyltransferase. Journal of Structural Biology 116(2): 330-334, 1996

Crystallization and preliminary crystallographic studies of antibacterial polypeptide LCI expressed in Escherichia coli. Acta Crystallographica Section D Biological Crystallography 57(12): 1931-1932, 2001

Crystallization and preliminary crystallographic studies of glucosamine-6-phosphate deaminase from Escherichia coli K12. Journal of Molecular Biology 226(4): 1283-1286, 1992

Crystallization and preliminary X-ray crystallographic studies of 7alpha-hydroxysteroid dehydrogenase from Escherichia coli. Acta Crystallographica. Section D, Biological Crystallography 52(Pt 1): 215-217, 1996

Crystallization and preliminary crystallographic studies of Escherichia coli CcmG/DsbE protein. Acta Crystallographica Section D Biological Crystallography 59(9): 1674-1675, 2003

Crystallization and preliminary crystallographic studies of a new L-asparaginase encoded by the Escherichia coli genome. Acta Crystallographica Section D Biological Crystallography 56(11): 1505-1507, 2000