+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

DNA sequencing research group: 2006 general survey of DNA sequencing facilities

DNA sequencing research group: 2006 general survey of DNA sequencing facilities

Journal of Biomolecular Techniques 18(2): 113-119

Over the past few years, technological advances in automated DNA sequencing have had a profound effect on the nature of DNA sequencing laboratories. To characterize the changes occurring within DNA sequencing facilities, the DNA Sequencing Research Group conducted three previous studies, in 1998, 2000, and 2003. A new general survey has been designed and conducted by the DSRG to capture the current status of DNA sequencing facilities in all sectors. Included were questions regarding facility administration, pricing, instrumentation, technology, protocols, and operation. The results of the survey are presented here, accompanied by comparisons to the previous surveys. These comparisons formed a basis for the discussion of trends within the facilities in response to the dynamics of a changing technology.

(PDF emailed within 1 workday: $29.90)

Accession: 052441793

Download citation: RISBibTeXText

PMID: 17496223

Related references

PIMS sequencing extension: a laboratory information management system for DNA sequencing facilities. Bmc Research Notes 4(): 48-48, 2011

Quality control of DNA sequencing templates and contig assembly using quantitative DNA fiber mapping facilities parallelism in producing sequencing. American Journal of Human Genetics 59(4 SUPPL ): A314, 1996

Identification of optimal protocols for sequencing difficult templates: results of the 2008 ABRF DNA Sequencing Research Group difficult template study 2008. Journal of Biomolecular Techniques 20(2): 116-127, 2009

Multiple group-specific sequencing primers for reliable and rapid DNA sequencing. Molecular & Cellular Probes 17(4): 203-210, August, 2003

SeqSharp: A general approach for improving cycle-sequencing that facilitates a robust one-step combined amplification and sequencing method. Journal of Molecular Diagnostics 12(3): 272-277, 2010

Genome survey sequencing and genetic background characterization of Gracilariopsis lemaneiformis (Rhodophyta) based on next-generation sequencing. Plos One 8(7): E69909-E69909, 2014

Revealing the results of whole-genome sequencing and whole-exome sequencing in research and clinical investigations: some ethical issues. Journal of Medical Ethics 41(4): 317-321, 2015

Sequencing at sea: challenges and experiences in Ion Torrent PGM sequencing during the 2013 Southern Line Islands Research Expedition. Peerj 2: E520-E520, 2014

Discrimination of the Lactobacillus acidophilus group using sequencing, species-specific PCR and SNaPshot mini-sequencing technology based on the recA gene. Journal of the Science of Food and Agriculture 92(13): 2703-2708, 2013

Rapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers. Nucleic Acids Research 21(14): 3333-3334, 1993

Two novel sequencing strategies SimulSeq, simultaneous sequencing of multiple PCR products; and AmpliSeq, combined PCR amplification and cycle sequencing in single reactions. American Journal of Pathology 161(1): 27-33, July, 2002

Sequencing of 2 subclinical atherosclerosis candidate regions in 3669 individuals: Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) Consortium Targeted Sequencing Study. Circulation. Cardiovascular Genetics 7(3): 359-364, 2015

Impact of Next Generation Sequencing on the Organization and Funding of Returning Research Results: Survey of Canadian Research Ethics Boards Members. Plos One 11(5): E0154965-E0154965, 2016

Targeted sequencing in candidate genes for atrial fibrillation: the Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) Targeted Sequencing Study. Heart Rhythm 11(3): 452-457, 2014