+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Demonstration of immunoglobulin m on avian lymphoid leukosis lymphoma cells by the unlabelled antibody peroxidase-antiperoxidase method



Demonstration of immunoglobulin m on avian lymphoid leukosis lymphoma cells by the unlabelled antibody peroxidase-antiperoxidase method



Avian Pathology 16(2): 253-268



A series of 12 avian lymphoid leukosis and nine Marek's disease lymphomas from experimental and natural infections were studied for the demonstration of immunoglobulin M and Marek's disease tumour-associated surface antigen by the unlabelled antibody peroxidase-antiperoxidase method using impression smears and formaldehyde-fixed paraffin-embedded tissue sections. Results indicate that immunoglobulin M, the characteristic antigenic marker of avian lymphoid leukosis virus-induced B cell lymphomas, can be detected not only on tumour imprints but also in deparaffinised tissue sections. Of 12 chickens with proven lymphoid leukosis lymphomas, 10 were clearly diagnosed by demonstration of a predominance of immunoglobulin M-bearing cells in the lesions. Negative results of samples from two birds were due to unknown reasons or to post mortem deterioration of tissues. This technique is compatible with the routine histological tissue-processing, thus providing a specific complementary diagnostic tool for the identification of lymphoid leukosis lymphomas. Marek's disease tumour-associated surface antigen, the specific antigenic surface marker of Marek's disease tumour cells, was demonstrated only in one case using tumour imprints, but in no case with paraffin sections. This could possibly be due to the low frequency of this antigen in the lesions or to the loss of its antigenicity during the procedure used.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052483068

Download citation: RISBibTeXText

PMID: 18766612

DOI: 10.1080/03079458708436373


Related references

On the application of the unlabelled peroxidase-antiperoxidase method to the investigations of the nervous tissue. Folia Histochemica et Cytochemica 21(1): 67-73, 1983

The unlabelled-peroxidase antiperoxidase method used for the localization of leghaemoglobin in Glycine max nodules. Archives Internationales de Physiologie et de Biochimie 87(3): 637-638, 1979

Demonstration of secretory component immunoglobulin a and immunoglobulin m by the peroxidase antiperoxidase technique in inverted papillomas of the nasal cavities. Human Pathology 15(10): 915-920, 1984

3H-TdR labeling index measurement of immunoglobulin-containing cells by combination of autoradiography with peroxidase-antiperoxidase method. Nihon Ketsueki Gakkai Zasshi 49(7): 1432-1435, 1986

The demonstration of proliferating cells by using monoclonal antibodies to 5-bromo-2'-deoxyuridine in the peroxidase-antiperoxidase method. Tsitologiia 32(12): 1225-1230, 1990

Tritiated thymidine labeling index measurement of immunoglobulin containing cells by combination of autoradiography with peroxidase antiperoxidase method. Acta Haematologica Japonica 49(7): 1432-1435, 1986

Identification of prolactin cells in a mammosomatotropic tumor by the unlabeled-antibody peroxidase-antiperoxidase method. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 145(2): 524-527, 1974

Rapid induction of lymphoid leukosis and ascites by avian leukosis virus from a lymphoid leukosis cell line. Journal of Veterinary Medical Science 60(1): 77-85, 1998

Multilayer peroxidase-labeled antibody method: Comparison with labeled streptavidin-biotin method, avidin-biotin-peroxidase complex method, and peroxidase-antiperoxidase method. Journal of Clinical Laboratory Analysis 9(6): 424-430, 1995

Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material. Histochemistry 43(3): 291-294, 1975

Standardization of a peroxidase-antiperoxidase method for the demonstration of HBsAg in liver sections. Pathologica 73(1024): 241-245, 1981

Demonstration of cell surface antigens and their antibodies by the peroxidase-antiperoxidase method. Transplantation 25(6): 331-334, 1978