+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil



Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil



Brazilian Journal of Microbiology 44(3): 693-699



Campylobacteriosis is a worldwide distributed zoonosis. One of the main virulence factors related to Campylobacter spp. in animals and humans is the cytolethal distending toxin (CDT), encoded by three adjacent genes (cdtA, cdtB, cdtC). The occurrence of Campylobacter spp. in samples of vegetables has not been reported in Brazil yet, and has seldom been described in the international literature. The detection of CDT in these strains has not been reported, either. The objectives of the present study were to determine the occurrence of Campylobacter spp. strains carrying virulence factors in samples of poultry and vegetables (lettuce and spinach) from different points of sale, thus verifying if vegetables are as an important vehicle for potentially virulent Campylobacter spp. strains as poultry. Twenty four strains were identified as Campylobacter jejuni by phenotypic and genotypic methods: 22 from broiler carcasses and two from lettuce samples. Three strains were identified as Campylobacter coli: two from broiler carcasses and one from lettuce. The presence of the cdt genes were detected in 20/24 (83.3%) C. jejuni strains, and 3/3 (100%) C. coli strains. The isolation of Campylobacter spp. strains with the cdt gene cluster in lettuce samples points to a new possible source of contamination, which could have an impact in the vegetable production chain and risk to public health. Results show that potentially virulent C. jejuni and C. coli strains remain viable in samples of broiler carcasses and vegetables at the points of sale.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 052516470

Download citation: RISBibTeXText

PMID: 24516435


Related references

Detection of Cdt toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in So Paulo, Brazil. Brazilian Journal of Microbiology 44(3): 693-699, 2013

Detection of cytolethal distending toxin activity and cdt genes in Campylobacter spp. isolated from chicken carcasses. Applied and Environmental Microbiology 65(4): 1501-1505, 1999

Detection and analysis of Cytolethal Distending Toxin (CDT) genes in Campylobacter jejuni and Camplyobacter coli isolates by polymerase chain reaction and determination of the prevalence of CDT genes and CDT activity in campylobacters isolated from chicken carcasses. 1998

Campylobacter spp isolated from chicken carcasses Prevalence and detection of cytolethal distending toxin production. Abstracts of the General Meeting of the American Society for Microbiology 98: 413, 1998

Shiga toxin and beta-lactamases genes in Escherichia coli phylotypes isolated from carcasses of broiler chickens slaughtered in Iran. International Journal of Food Microbiology 177: 16-20, 2014

Prevalence of virulence genes in strains of Campylobacter jejuni isolated from human, bovine and broiler. Brazilian Journal of Microbiology 44(4): 1223-1229, 2015

Incidence of the virulence markers iam in Campylobacter jejuni and Campylobacter coli strains isolated from poultry carcasses. Roczniki Panstwowego Zakadu Higieny 55(4): 307-312, 2004

Nonproduction of cyto toxin among entero pathogenic strains of escherichia coli isolated in sao paulo brazil. Revista de Microbiologia 14(3): 161-162, 1983

Production of shiga like toxin among escherichia coli strains and other bacteria isolated from diarrhea in sao paulo brazil. Journal of Clinical Microbiology 28(6): 1460-1462, 1990

Prevalence of 11 pathogenic genes of Campylobacter jejuni by PCR in strains isolated from humans, poultry meat and broiler and bovine faeces. Journal of Medical Microbiology 52(Pt 4): 345-348, 2003

Occurrence of Salmonella spp in broiler carcasses from slaughter plants of Sao Paulo, Brazil. Ciencia Rural: 9, 2557-2560, 2008

Phenotypic and genotypic characteristics of shiga toxin-producing Escherichia coli strains isolated from children in São Paulo, Brazil. Memorias do Instituto Oswaldo Cruz 97(8): 1085-1089, 2003

Phenotypic and genotypic characteristics of shiga toxin-producing Escherichia coli strains isolated from children in So Paulo, Brazil. Memórias do Instituto Oswaldo Cruz 97(8): 1085-1089, 2002

Molecular subtyping of Campylobacter jejuni subsp. jejuni strains isolated from different animal species in the state of Sao Paulo, Brazil. Brazilian Journal of Microbiology 36(4): 378-382, 2005

Biotype and Lior's serogroup of campylobacter jejuni and campylobacter coli isolated in Sao Paulo, Brazil. Revista de Microbiologia 25(1): 1-5, 1994