EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Detection of Caprine-specific Nucleic Acid Sequences in Goat Milk Using Polymerase Chain Reaction



Detection of Caprine-specific Nucleic Acid Sequences in Goat Milk Using Polymerase Chain Reaction



Materia Socio-Medica 25(2): 105-108



none declared. This study was carried out to evaluate PCR-based method for detection of DNA in goat milk. It utilized primers targeting the mitochondrial cytochrome -b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. For the specific identification of goat mtcyt-b gene, pair of primers (GSL1, GSR2), were used, which produced a 428 base pair (bp) PCR product from milk samples as well as from peripheral blood. Amplification products were visualized on ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR was applied to DNA from animal species including cattle, sheep, swine, camel, deer, horse, donkey, and human, which indicates that the 2 pairs of primers are specific for goat. DNA can be extracted from goat milk and would be advantageous in the variety of application such as species identification in milk and milk.

(PDF emailed within 0-6 h: $19.90)

Accession: 052516523

Download citation: RISBibTeXText

PMID: 24493993

DOI: 10.5455/msm.2013.25.105-108



Related references

Specific detection of small ruminant lentiviral nucleic acid sequences located in the proviral long terminal repeat and leader-gag regions using real-time polymerase chain reaction. Journal of Virological Methods 147(2): 338-344, 2007

Quantitative method for specific nucleic acid sequences using competitive polymerase chain reaction with an alternately binding probe. Analytical Chemistry 79(3): 974-979, 2007

Does hiv 1 persist in wastewater? detection of hiv 1 specific nucleic acid using polymerase chain reaction. Abstracts of the General Meeting of the American Society for Microbiology 92: 362, 1992

Detection of the cow's milk component in ewe's and goat's cheese using polymerase chain reaction. Bulletin Potravinarskeho Vyskumu 41(3): 163-167, 2002

Polymerase chain reaction (PCR) for detection of Brucella melitensis in goat milk. Small Ruminant Research 65(1-2): 79-84, 2006

Polymerase chain reaction (PCR) search for viral nucleic acid sequences in schizophrenia. British Journal of Psychiatry 166(1): 55-60, 1995

Rapid method for preferential coamplification of two different nucleic acid sequences using polymerase chain reaction. Official Gazette of the United States Patent & Trademark Office Patents 1203(1): 425, Oct 7, 1997

Peptide nucleic acid-locked nucleic acid polymerase chain reaction clamp-based detection test for gefitinib-refractory T790M epidermal growth factor receptor mutation. Cancer Science 99(3): 595-600, 2008

Double-nested polymerase chain reaction for detection of caprine arthritis-encephalitis virus proviral DNA in blood, milk, and tissues of infected goats. Journal of Virological Methods 50(1-3): 101-113, 1994

DNA sequences for the specific detection of Cryptosporidium parvum by the polymerase chain reaction. American Journal of Tropical Medicine and Hygiene 45(6): 688-694, 1991

Significance of the polymerase chain reaction pcr assay in the detection of hcv specific sequences. Hepatology 12(4 PART 2): 952, 1990

Search for viral nucleic acid sequences in brain tissues of patients with schizophrenia using nested polymerase chain reaction. Archives of General Psychiatry 53(1): 32-40, 1996

Simultaneous detection of hcv and hiv 1 specific sequences in a single polymerase chain reaction assay. Hepatology 12(4 PART 2): 951, 1990

Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation. Official Gazette of the United States Patent & Trademark Office Patents 1283(1), June 1, 2004

Detection of nucleic acid of HRV by a Dig-labelled probe prepared with polymerase chain reaction (PCR). Virologica Sinica 10(1): 22-27, 1995