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Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction



Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction



Euro Surveillance 17(39):



We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

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Accession: 052518080

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PMID: 23041020


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