+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of carbapenemase encoding genes in Enterobacteriace, Pseudomonas aeruginosa, and Acinetobacter baumanii isolated from patients at Intensive Care Unit Cipto Mangunkusumo Hospital in 2011



Detection of carbapenemase encoding genes in Enterobacteriace, Pseudomonas aeruginosa, and Acinetobacter baumanii isolated from patients at Intensive Care Unit Cipto Mangunkusumo Hospital in 2011



Acta Medica Indonesiana 45(2): 101-106



to determine the prevalence of carbapenemase encoding genes (blaIMP-1, blaVIM-2, blaKPC-2, blaOXA-48, and blaNDM-1) of carbapenem-resistant Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumanii isolated from the intensive care unit patients as pathogens, in Cipto Mangunkusumo hospital (ICU-RSCM) in 2011. we examined the carbapenemase encoding genes in the clinical microbiology laboratory (LMK FKUI/RSCM). Duplex- and simplex PCR methods were conducted to detect the resistant genes. we found 4 (5%) P. aeruginosa strains carry blaIMP-1 gene and all were isolated from sputum specimens. The prevalence of carbapenem resistant among Gram-negative bacilli isolated from ICU-RSCM, are Enterobacteriaceae 27.6%, P. aeruginosa 21.9%, and A. baumannii 50.5%. The New Delhi Metallo--lactamase encoding gene (blaNDM-1) was detected in 1 K. pneumonia isolated from sputum as well. The other genes, i.e. blaKPC-2, blaVIM-2, and blaOXA-48 were not found in any isolates. The absence of other genes indicated that other mechanisms may play a role in the occurrence of carbapenem resistance in pathogens isolated in ICU-RSCM. this study confirmed that the prevalence of carbapenems resistant Gram-negative bacilli in ICU-RSCM in 2011 was high. The carbapenemase encoding genes, which were detected among the carbapenems resistant Gram-negative bacilli, were blaIMP-1 and blaNDM-1.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 052519142

Download citation: RISBibTeXText

PMID: 23770789


Related references

Phenotype characterization of Beta-lactamase producing enterobacteriaceae in the intensive care unit (ICU) of Cipto Mangunkusumo Hospital in 2011. Acta Medica Indonesiana 45(1): 11-16, 2013

Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospital. Journal of Laboratory Physicians 10(2): 208-213, 2018

Optimal Amikacin Levels for Patients with Sepsis in Intensive Care Unit of Cipto Mangunkusumo Hospital, Jakarta, Indonesia. Acta Medica Indonesiana 49(3): 227-235, 2017

The genetic description of Acinetobacter baumannii and Pseudomonas aeruginosa strains isolated from patients with hospital acquired pneumonia hospitalized in intensive care unit. Medycyna Doswiadczalna i Mikrobiologia 62(1): 67-75, 2010

Molecular detection of integron genes and pattern of antibiotic resistance in pseudomonas aeruginosa strains isolated from intensive care unit, shahid beheshti hospital, north of iran. International Journal of Molecular and Cellular Medicine 1(4): 209-217, 2012

Detection of carbapenemase-encoding genes among clinical isolates of Pseudomonas aeruginosa in a Chinese burn unit. Journal of Burn Care and Research 34(4): 453-458, 2014

DNA amplification fingerprinting (DAF) applied to the investigation of Acinetobacter baumanii isolated from intensive care unit patients. Irish Journal of Medical Science 165(1): 44-48, 1996

Metallo-beta-lactamase-producing clinical isolates of Acinetobacter species and Pseudomonas aeruginosa from intensive care unit patients of a tertiary care hospital. Indian Journal of Medical Microbiology 26(3): 243-245, 2008

Acinetobacter baumannii: Role in Blood Stream Infection in Neonatal Unit, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia. International Journal of Microbiology 2013: 180763, 2013

Changes in resistance percentage to antibiotics in Pseudomonas aeruginosa and Acinetobacter baumannii strains isolated from blood cultures of intensive care unit patients. Turkish Bulletin of Hygiene and Experimental Biology 71(1): 1-8, 2014

Resistance pattern and distribution of carbapenemase and antiseptic resistance genes among multidrug-resistant Acinetobacter baumannii isolated from intensive care unit patients. Journal of Medical Microbiology 67(10): 1467-1473, 2018

Spread of multidrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa clones in patients with ventilator-associated pneumonia in an adult intensive care unit at a university hospital. Brazilian Journal of Infectious Diseases 19(4): 350-357, 2016

Four years of monitoring of antibiotic sensitivity rates of Pseudomonas aeruginosa and Acinetobacter baumannii strains isolated from patients in intensive care unit and inpatient clinics. Mikrobiyoloji Bulteni 42(2): 321-329, 2008

Dissemination of a class I integron carrying VIM-2 carbapenemase in Pseudomonas aeruginosa clinical isolates from a hospital intensive care unit in Annaba, Algeria. Antimicrobial Agents and ChemoTherapy 57(5): 2426-2427, 2013

Drug resistance of Acinetobacter baumanii isolated from children in the pediatric intensive care unit. Zhongguo Dang Dai Er Ke Za Zhi 14(3): 229-230, 2012