+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of micrometastasis in lymph nodes of oral squamous cell carcinoma: A comparative study

Detection of micrometastasis in lymph nodes of oral squamous cell carcinoma: A comparative study

Journal of Oral and Maxillofacial Pathology 17(3): 374-380

The annual mortality rate from head and neck squamous cell carcinoma (HNSCC) is over 11,000 worldwide. Squamous cell carcinoma of the head and neck (SCCHN) frequently metastasizes to the regional lymph nodes which are the first site of arrest of tumor cells that have invaded the peritumoral lymphatics, hence the strongest predictor of disease prognosis and outcome. The present study aims to compare the efficacy of frozen sections (cryosection), step-serial sectioning conventional H and E staining, immunohistochemistry (IHC) and RT-PCR analysis in detection of lymph node micrometastasis. A prospective series of 30 patients who were diagnosed with primary squamous cell carcinoma of the oral cavity and underwent surgical treatment including unilateral or bilateral selective neck dissection were considered for the study. Metastatic carcinomatous cells were observed in H and E staining of frozen section in 18 lymph nodes (54%) and in 19 lymph nodes (57%) in step-serial sectioned H and E-stained sections of the 78 lymph nodes from 30 patients. Carcinomatous cells were immunolabeled with pancytokeratin in 18 lymphnodes (54%). CK19 mRNA was detected in 33 lymph nodes of 16 patients. RT-PCR gave positive signals for 24% and 23% of lymph nodes positive by histopathology and immunohistochemistry. Our study demonstrated that RT-PCR is far more sensitive in detection of micrometastasis than any other technique used in routine procedures and immunohistochemistry. Fifty-three percent patients with micrometastasis detected by RT-PCR had large T3/T4 tumors. Prognosis was poor for patients who were positive for micrometastasis detected only by RT-PCR, among which two patients died within a period of 6 months.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 052522026

Download citation: RISBibTeXText

PMID: 24574655

Related references

Detection of cervical lymph node micrometastasis and isolated tumor cells in oral squamous cell carcinoma using immunohistochemistry and serial sectioning. Journal of Oral and Maxillofacial Pathology 20(3): 436-444, 2016

Immunohistochemically detected micrometastasis in lymph nodes from superficial esophageal squamous cell carcinoma. Journal of Surgical Oncology 82(3): 153-159, 2003

Preoperative evaluation of cervical lymph nodes for metastasis in patients with oral squamous cell carcinoma: A comparative study of efficacy of palpation, ultrasonography and computed tomography. National Journal of Maxillofacial Surgery 7(2): 186-190, 2017

Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma - A Histochemical Study. Journal of Clinical and Diagnostic Research 10(12): Zc23-Zc26, 2017

The relationship between lymphangiogenesis and cervical lymph node micrometastasis in oral squamous cell carcinoma. Zhonghua Kou Qiang Yi Xue Za Zhi 39(3): 221-223, 2004

A Novel Multimodal NIR-II Nanoprobe for the Detection of Metastatic Lymph Nodes and Targeting Chemo-Photothermal Therapy in Oral Squamous Cell Carcinoma. Theranostics 9(2): 391-404, 2019

Clinical value of genetically diagnosed lymph node micrometastasis for patients with oral squamous cell carcinoma. Head & Neck 27(8): 676-681, 2005

Micrometastasis and tumor cell microinvolvement of lymph nodes from esophageal squamous cell carcinoma: Frequency, associated tumor characteristics, and impact on prognosis. Cancer 83(5): 858-866, 1998

An analysis of cervical lymph nodes metastasis in oral squamous cell carcinoma. Relationship between grade of histopathological malignancy and lymph nodes metastasis. International Journal of Oral and Maxillofacial Surgery 32(3): 284-288, 2003

Expression of E-cadherin in primary oral squamous cell carcinoma and metastatic lymph nodes: an immunohistochemical study. Indian Journal of Dental Research 20(1): 71-76, 2009

Clinical significance of ultrasonographic examination including detection of thyroid gland diseases when surveying cervical lymph nodes in subjects with oral squamous cell carcinoma. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics 109(6): E78-E85, 2010

Study of the influence of nodal liquefaction in measurement of the ADC value of OSCC metastatic lymph nodes in patients with oral squamous cell carcinoma. Shanghai Kou Qiang Yi Xue 24(1): 98-101, 2015

Preoperative [18F]fluorodeoxyglucose positron emission tomography standardized uptake value of neck lymph nodes predicts neck cancer control and survival rates in patients with oral cavity squamous cell carcinoma and pathologically positive lymph nodes. International Journal of Radiation Oncology, Biology, Physics 74(4): 1054-1061, 2009

Metastasis to regional lymph nodes in patients with esophageal squamous cell carcinoma: CT versus FDG PET for presurgical detection prospective study. Radiology 227(3): 764-770, 2003

Usefulness of 3T diffusion-weighted MRI for discrimination of reactive and metastatic cervical lymph nodes in patients with oral squamous cell carcinoma: a pilot study. Dento Maxillo Facial Radiology 43(3): 20130202, 2014