+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Development of microfluidic-based heterogeneous immunoassays



Development of microfluidic-based heterogeneous immunoassays



Frontiers in Bioscience 2: 73-84



Microfluidic-based heterogeneous immunoassays are reviewed in this article by first introducing the principle of immunoassay, followed by a discussion of microfluidic-based technology. Microfabrication, surface modification, solution dispensing and detection technology are discussed and their applications to biomolecular detection reviewed. In the future, improved manufacturing processes and integrated assay systems in an automatic fashion with a reduced assay time and reagent consumption will allow for the effective detection of biomolecules that are of interest in medical diagnostics, drug discovery and bioterrorism.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052562142

Download citation: RISBibTeXText

PMID: 20036929

DOI: 10.2741/s46


Related references

Effect of volume- and time-based constraints on capture of analytes in microfluidic heterogeneous immunoassays. Lab on a Chip 8(12): 2062-2070, 2008

A digital microfluidic approach to heterogeneous immunoassays. Analytical and Bioanalytical Chemistry 399(1): 337-345, 2011

Heterogeneous immunoassays using magnetic beads on a digital microfluidic platform. Lab on a Chip 8(12): 2188-2196, 2008

Electrokinetically driven microfluidic chips with surface-modified chambers for heterogeneous immunoassays. Analytical Chemistry 73(14): 3400-3409, 2001

Heterogeneous immunoassays in microfluidic format using fluorescence detection with integrated amorphous silicon photodiodes. Biomicrofluidics 5(1): 14102, 2011

Quantitative heterogeneous immunoassays in protein modified polydimethylsiloxane microfluidic channels for rapid detection of disease biomarkers. Methods in Molecular Biology 949: 335-347, 2013

Bead-based microfluidic immunoassays: the next generation. Biosensors and Bioelectronics 22(7): 1197-1204, 2007

Microfluidics-Based Biosensors: A Microfluidic Paper-Based Origami Nanobiosensor for Label-Free, Ultrasensitive Immunoassays (Adv. Healthcare Mater. 11/2016). Advanced Healthcare Materials 5(11): 1378, 2016

Digital microfluidic magnetic separation for particle-based immunoassays. Analytical Chemistry 84(20): 8805-8812, 2012

Towards an electrowetting-based digital microfluidic platform for magnetic immunoassays. Lab on a Chip 9(23): 3433-3436, 2009

Microfluidic immunoassays as rapid saliva-based clinical diagnostics. Proceedings of the National Academy of Sciences of the United States of America 104(13): 5268-5273, 2007

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays. Journal of Visualized Experiments 2017(121):, 2017

Microfluidic device for immunoassays based on surface plasmon resonance imaging. Lab on a Chip 8(5): 694-700, 2008

Modeling and optimization of high-sensitivity, low-volume microfluidic-based surface immunoassays. Biomedical Microdevices 7(2): 99, 2005

User configurable microfluidic device for multiplexed immunoassays based on DNA-directed assembly. Analytical Chemistry 81(3): 1275-1279, 2009