+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Diagnostic segregation of human brain tumours using Fourier-transform infrared and/or Raman spectroscopy coupled with discriminant analysis



Diagnostic segregation of human brain tumours using Fourier-transform infrared and/or Raman spectroscopy coupled with discriminant analysis



Analytical Methods 5: 89-102



The most common initial treatment received by patients with a brain tumour is surgical removal of the growth. Precise histopathological diagnosis of brain tumours is to some extent subjective. Furthermore, currently available diagnostic imaging techniques to delineate the excision border during cytoreductive surgery lack the required spatial precision to aid surgeons. We set out to determine whether infrared (IR) and/or Raman spectroscopy combined with multivariate analysis could be applied to discriminate between normal brain tissue and different tumour types (meningioma, glioma and brain metastasis) based on the unique spectral "fingerprints" of their biochemical composition. Formalin-fixed paraffin-embedded tissue blocks of normal brain and different brain tumours were de-waxed, mounted on low-E slides and desiccated before being analyzed using attenuated total reflection Fourier-transform IR (ATR-FTIR) and Raman spectroscopy. ATR-FTIR spectroscopy showed a clear segregation between normal and different tumour subtypes. Discrimination of tumour classes was also apparent with Raman spectroscopy. Further analysis of spectral data revealed changes in brain biochemical structure associated with different tumours. Decreased tentatively-assigned lipid-to-protein ratio was associated with increased tumour progression. Alteration in cholesterol esters-to-phenylalanine ratio was evident in grade IV glioma and metastatic tumours. The current study indicates that IR and/or Raman spectroscopy have the potential to provide a novel diagnostic approach in the accurate diagnosis of brain tumours and have potential for application in intra-operative diagnosis.

(PDF emailed within 0-6 h: $19.90)

Accession: 052587368

Download citation: RISBibTeXText

PMID: 24098310

DOI: 10.1039/C2AY25544H


Related references

Segregation of human prostate tissues classified high-risk (UK) versus low-risk (India) for adenocarcinoma using Fourier-transform infrared or Raman microspectroscopy coupled with discriminant analysis. Analytical and Bioanalytical Chemistry 401(3): 969-982, 2011

A comparison of fourier transform infrared and near infrared fourier transform raman spectroscopy for quantitative measurements an application in polymorphism. Spectrochimica Acta Part A Molecular Spectroscopy 47(9-10): 1217-1224, 1991

A comparison of Fourier transform infrared and near-infrared Fourier transform Raman spectroscopy for quantitative measurements: An application in polymorphism. Spectrochimica Acta Part A: Molecular Spectroscopy 47(9-10): 1217-1223, 1991

Fourier-transform infrared spectroscopy coupled with a classification machine for the analysis of blood plasma or serum: a novel diagnostic approach for ovarian cancer. Analyst 138(14): 3917-3926, 2013

Rapid and non-destructive identification of calligraphies by Fourier transform infrared spectroscopy and Fourier transform Raman spectroscopy. Guang Pu Xue Yu Guang Pu Fen Xi 24(11): 1327-1330, 2005

Fourier-Transform Raman and Fourier-Transform Infrared Spectroscopy (An Investigation of Five Higher Plant Cell Walls and Their Components). Plant Physiology 106(4): 1623-1631, 1994

Utility of Fourier transform-Raman and Fourier transform-infrared diffuse reflectance spectroscopy for differentiation of polymorphic spironolactone samples. Journal of Pharmaceutical Sciences 81(12): 1141-1146, 1992

Differentiating hard from soft woods using fourier transform infrared and fourier transform raman spectroscopy. Spectrochimica Acta Part A Molecular Spectroscopy 47(9-10): 1441-1448, 1991

Identification of natural dyes on laboratory-dyed wool and ancient wool, silk, and cotton fibers using attenuated total reflection (ATR) Fourier transform infrared (FT-IR) spectroscopy and Fourier transform Raman spectroscopy. Applied Spectroscopy 65(9): 1017-1023, 2011

Near infrared fourier transform Raman spectroscopy of human artery. Spectrochimica Acta Part A: Molecular Spectroscopy 47(3-4): 509-512, 1991

Fourier transform raman spectroscopy using a bench-top fourier transform infrared spectrometer. Microchimica Acta 95(1-6): 231-234, 1988

Forensic classification of black inkjet prints using Fourier transform near-infrared spectroscopy and Linear Discriminant Analysis. Forensic Science International 299: 128-134, 2019

Near-infrared Fourier transform Raman spectroscopy in human lens research. Experimental Eye Research 51(5): 619-623, 1990

Diagnosis of Periodontal Disease from Saliva Samples Using Fourier Transform Infrared Microscopy Coupled with Partial Least Squares Discriminant Analysis. Analytical Sciences 32(2): 225-231, 2016

Penalized discriminant analysis for the detection of wild-grown and cultivated Ganoderma lucidum using Fourier transform infrared spectroscopy. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy 159: 68-77, 2016