+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Different expression of Sox9 and Runx2 between chondrosarcoma and dedifferentiated chondrosarcoma cell line



Different expression of Sox9 and Runx2 between chondrosarcoma and dedifferentiated chondrosarcoma cell line



European Journal of Cancer Prevention 19(6): 466-471



Dedifferentiated chondrosarcoma (DDCS), a highly malignant variant of chondrosarcoma (CCS), is characterized by high-grade sarcoma adjacent to low-grade chondroid tumor. Owing to its complicated composition, the histogenesis of this tumor remains controversial. Earlier, we carried out DNA microarray analysis using chondrosarcomatous tissues, and found that Sox9 and runt-related transcription factor 2 (Runx2) were differentially expressed in CCS compared with DDCS. Here, we analyzed Sox9, Runx2, Col2a1, and Col1a1 in NDCS-1 (DDCS) and SW1353 (CCS) cell lines using reverse transcriptase-polymerase chain reaction, western blot, and immunocytochemistry. The results showed high expression of Runx2 and Col1a1, and low expression of Sox9 and Col2a1 in NDCS-1 cells. In SW1353 cells, however, gene expressions were reversed. These findings provide evidence that Sox9 and Runx2 are involved in the occurrence and development of DDCS.

(PDF emailed within 0-6 h: $19.90)

Accession: 052605111

Download citation: RISBibTeXText

PMID: 20683191

DOI: 10.1097/CEJ.0b013e32833d942f


Related references

Dedifferentiated chondrosarcoma mimicking a giant cell tumor. Is this low grade dedifferentiated chondrosarcoma?. Pathology, Research and Practice 210(3): 194-197, 2014

BMPR2-pSMAD1/5 signaling pathway regulates RUNX2 expression and impacts the progression of dedifferentiated chondrosarcoma. American Journal of Cancer Research 6(6): 1302-1316, 2016

Three new chondrosarcoma cell lines: one grade III conventional central chondrosarcoma and two dedifferentiated chondrosarcomas of bone. Bmc Cancer 12: 375, 2013

A novel mutated cell line with characteristics of dedifferentiated chondrosarcoma. International Journal of Molecular Medicine 24(4): 427-435, 2009

Establishment and characterization of a novel dedifferentiated chondrosarcoma cell line, NCC-dCS1-C1. Human Cell 2019, 2019

Chondrosarcoma with additional mesenchymal component (dedifferentiated chondrosarcoma). I. A clinicopathologic study of 26 cases. Cancer 58(2): 278-286, 1986

Chondrosarcoma of the larynx with additional malignant mesenchymal component (dedifferentiated chondrosarcoma). American Journal of Surgical Pathology 12(4): 314-320, 1988

Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation. Virchows Archiv 451(3): 691-699, 2007

Chondrosarcoma with additional mesenchymal component (dedifferentiated chondrosarcoma). II. An immunohistochemical and electron microscopic study. Cancer 58(2): 287-298, 1986

Juxtacortical dedifferentiated chondrosarcoma from a primary periosteal chondrosarcoma. Modern Pathology 9(3): 279-283, 1996

Chondrosarcoma of the urinary bladder and establishment of a human chondrosarcoma cell line (OCUU-6). Human Cell 17(2): 93-96, 2004

Outcome of First-Line Systemic Treatment for Unresectable Conventional, Dedifferentiated, Mesenchymal, and Clear Cell Chondrosarcoma. Oncologist 2018, 2018

Dedifferentiated chondrosarcoma: an aggressive variant of chondrosarcoma. Asian Cardiovascular and Thoracic Annals 23(2): 221-223, 2015

Is dedifferentiated chondrosarcoma a 'de-differentiated' chondrosarcoma?. Journal of Pathology 189(4): 445-447, 2000

Case report 347. Dedifferentiated chondrosarcoma: grade 4 fibrosarcoma arising in grade 1 chondrosarcoma (femur). Skeletal Radiology 15(1): 77-81, 1986