+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Differential expression of ETS family members in prostate cancer tissues and androgen-sensitive and insensitive prostate cancer cell lines



Differential expression of ETS family members in prostate cancer tissues and androgen-sensitive and insensitive prostate cancer cell lines



International Journal of Molecular Medicine 28(1): 89-93



The ETS family of transcription factors plays important roles in both normal and neoplastic cells for different biological processes such as proliferation, differentiation, development, transformation, apoptosis, migration, invasion and angiogenesis. The 27 ETS factors are probably a part of complex regulatory networks including interactions among family members. In human prostate cancer, rearrangements have been found in several genes of the ETS family resulting in chimeric oncoproteins. In a previous study we found that the ETS family prototype, Ets-1 affects biological properties of PC3 prostate cancer cells. In a first effort to understand the cooperative interactions between different ETS factors in prostate cancer, in the present study we examined the expression pattern of all 27 ETS members using quantitative RT-PCR (qRT-PCR) in the androgen-sensitive VCaP and LNCaP, and the androgen-insensitive PC3 and DU-145 prostate cancer cell lines as well as in human prostate cancer tissue samples. We further investigated whether the ETS family prototype, Ets-1, regulates other ETS family members by examining the effect of Ets-1 blockade in PC3 cells on their expression. We found an expression specificity of various ETS family members in the prostate cancer cell lines which might reflect their different biological properties. In human prostate samples only 3 among the 27 ETS family members (Ehf, Elk-4 and Ets-2) showed significant expression differences between normal and cancerous prostate glands. We finally demonstrate that the family prototype, Ets-1, regulates the family members Elf-1, Elf-2, Elk-1, Etv-5 and Spi-1 in PC3 prostate cancer cells. Chimeric oncoproteins containing ETS family members arising due to frequent translocations in prostate cancer are probably part of a regulatory network involving other ETS family members as well.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052611099

Download citation: RISBibTeXText

PMID: 21491078

DOI: 10.3892/ijmm.2011.672


Related references

Epigenetics-related genes in prostate cancer: expression profile in prostate cancer tissues, androgen-sensitive and -insensitive cell lines. International Journal of Molecular Medicine 31(1): 21-25, 2013

Differential expression of interleukin-1 in androgen-sensitive and -insensitive prostate cancer cell lines. Proceedings of the American Association for Cancer Research Annual Meeting 40: 64, 1999

Differential effects of blueberry proanthocyanidins on androgen sensitive and insensitive human prostate cancer cell lines. Cancer Letters 231(2): 240-246, 2006

Differences in the expression and effects of interleukin-1 and -2 on androgen-sensitive and -insensitive human prostate cancer cell lines. Cancer Letters 149(1-2): 37-42, Feb 28, 2000

ERRgamma suppresses cell proliferation and tumor growth of androgen-sensitive and androgen-insensitive prostate cancer cells and its implication as a therapeutic target for prostate cancer. Cancer Research 67(10): 4904-4914, 2007

IL-6 causes multiple effects in androgen-sensitive and -insensitive prostate cancer cell lines. Expert Review of Endocrinology & Metabolism 6(3): 327-332, 2011

Mitogenic signaling in androgen sensitive and insensitive prostate cancer cell lines. Journal of Urology 163(3): 1027-1032, 2000

IL-6 causes multiple effects in androgen-sensitive and -insensitive prostate cancer cell lines. Expert Review of Endocrinology and Metabolism 6(3): 327-332, 2019

In vivo metabolism of testosterone in androgen-sensitive and -insensitive human prostate cancer cell lines. European Urology 36(5): 470, 1999

The role of caspase activation in the hypoxic response in both androgen sensitive and insensitive prostate cancer cell lines. Proceedings of the American Association for Cancer Research Annual Meeting 43: 875, 2002

Androgen sensitivity related proteins in hormone-sensitive and hormone-insensitive prostate cancer cell lines treated by androgen antagonist bicalutamide. Neoplasma 48(5): 419-424, 2002

Maspin expression is regulated by the non-canonical NF-κB subunit in androgen-insensitive prostate cancer cell lines. Molecular Immunology 49(1-2): 8-17, 2011

Nerve Growth Factor abrogates the tumorigenicity of androgen-insensitive prostate cancer cell lines Effect on telomerase activity, p75NGFR and androgen receptor expression. International Journal of Molecular Medicine 6(Suppl. 1): S43, 2000

Piperine blocks voltage gated K + current and inhibits proliferation in androgen sensitive and insensitive human prostate cancer cell lines. Archives of Biochemistry and Biophysics 2019, 2019

A differential ligand-mediated response of green fluorescent protein-tagged androgen receptor in living prostate cancer and non-prostate cancer cell lines. Journal of Histochemistry and Cytochemistry 55(6): 535-544, 2007