+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Differential responses of osteoblasts and macrophages upon Staphylococcus aureus infection



Differential responses of osteoblasts and macrophages upon Staphylococcus aureus infection



Bmc Microbiology 14: 207



Staphylococcus aureus (S. aureus) is one of the primary causes of bone infections which are often chronic and difficult to eradicate. Bacteria like S. aureus may survive upon internalization in cells and may be responsible for chronic and recurrent infections. In this study, we compared the responses of a phagocytic cell (i.e. macrophage) to a non-phagocytic cell (i.e. osteoblast) upon S. aureus internalization. We found that upon internalization, S. aureus could survive for up to 5 and 7 days within macrophages and osteoblasts, respectively. Significantly more S. aureus was internalized in macrophages compared to osteoblasts and a significantly higher (100 fold) level of live intracellular S. aureus was detected in macrophages compared to osteoblasts. However, the percentage of S. aureus survival after infection was significantly lower in macrophages compared to osteoblasts at post-infection days 1-6. Interestingly, macrophages had relatively lower viability in shorter infection time periods (i.e. 0.5-4 h; significant at 2 h) but higher viability in longer infection time periods (i.e. 6-8 h; significant at 8 h) compared to osteoblasts. In addition, S. aureus infection led to significant changes in reactive oxygen species production in both macrophages and osteoblasts. Moreover, infected osteoblasts had significantly lower alkaline phosphatase activity at post-infection day 7 and infected macrophages had higher phagocytosis activity compared to non-infected cells. S. aureus was found to internalize and survive within osteoblasts and macrophages and led to differential responses between osteoblasts and macrophages. These findings may assist in evaluation of the pathogenesis of chronic and recurrent infections which may be related to the intracellular persistence of bacteria within host cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052615482

Download citation: RISBibTeXText

PMID: 25059520

DOI: 10.1186/s12866-014-0207-5


Related references

Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection. Clinical and Diagnostic Laboratory Immunology 11(3): 463-472, 2004

Differential interactions of Streptococcus gordonii and Staphylococcus aureus with cultured osteoblasts. Molecular Oral Microbiology 28(4): 250-266, 2013

Monocyte chemoattractant protein-1 expression by osteoblasts following infection with Staphylococcus aureus or Salmonella. Journal of Interferon and Cytokine Research 21(5): 297-304, 2001

Activation of nuclear factor kappaB signaling pathway in human osteoblasts responses to Staphylococcus aureus in vitro. Zhonghua Wai Ke Za Zhi 50(3): 264-267, 2012

Differential innate immune responses of a living skin equivalent model colonized by Staphylococcus epidermidis or Staphylococcus aureus. Fems Microbiology Letters 290(2): 149-155, 2009

Differential induction of inflammatory cytokines and reactive oxygen species in murine peritoneal macrophages and resident fresh bone marrow cells by acute staphylococcus aureus infection: contribution of toll-like receptor 2 (TLR2). Inflammation 38(1): 224-244, 2015

TRAIL expression is induced in both osteoblasts containing intracellular Staphylococcus aureus and uninfected osteoblasts in infected cultures. Fems Microbiology Letters 278(2): 185-192, 2008

Staphylococcus aureus lipoproteins augment inflammatory responses in poly I:C-primed macrophages. Cytokine 111: 154-161, 2018

Prospective multicenter study of Staphylococcus aureus bacteremia - results from the "Invasive Staphylococcus aureus Infection Cohort" (INSTINCT) study FT Epidemiologie, Verlauf and Prognose der Staphylococcus-aureus-Bakteriamie - Erste Ergebnisse der INSTINCT-Kohorte. 2008

Staphylococcus aureus infection in various skin diseases: (2) Analysis of relation between Staphylococcus aureus infection and atopic dermatitis. Hifu 39(1): 29-32, 1997

Differential phagocytic response of polymorphonuclear leukocytes and alveolar macrophages to staphylococcus aureus and streptococcus pneumoniae in man and rabbits. Abstracts of the Annual Meeting of the American Society for Microbiology 75: 13, 1975

Staphylococcus aureus infection of mouse or human osteoblasts induces high levels of interleukin-6 and interleukin-12 production. Journal of Infectious Diseases 180(6): 1912-1920, 1999

The expression profiles of immune genes in Mus musculus macrophages during Staphylococcus aureus infection. Plos one 13(1): E0190490, 2018

Cytokine responses to Staphylococcus aureus bloodstream infection differ between patient cohorts that have different clinical courses of infection. Bmc Infectious Diseases 14: 580, 2014

Pyroptosis of resident macrophages differentially orchestrates inflammatory responses to Staphylococcus aureus in resistant and susceptible mice. European Journal of Immunology 45(3): 794-806, 2015