+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Differentiation of mesenchymal stem cells from human umbilical cord tissue into odontoblast-like cells using the conditioned medium of tooth germ cells in vitro



Differentiation of mesenchymal stem cells from human umbilical cord tissue into odontoblast-like cells using the conditioned medium of tooth germ cells in vitro



Biomed Research International 2013: 218543



The easily accessible mesenchymal stem cells in the Wharton's jelly of human umbilical cord tissue (hUCMSCs) have excellent proliferation and differentiation potential, but it remains unclear whether hUCMSCs can differentiate into odontoblasts. In this study, mesenchymal stem cells were isolated from the Wharton's jelly of human umbilical cord tissue using the simple method of tissue blocks culture attachment. UCMSC surface marker expression was then evaluated for the isolated cells using flow cytometry. The third-passage hUCMSCs induced by conditioned medium from developing tooth germ cells (TGC-CM) displayed high alkaline phosphatase (ALP) levels (P < 0.001), an enhanced ability to proliferate (P < 0.05), and the presence of mineralized nodules. These effects were not observed in cells treated with regular medium. After induction of hUCMSCs, the results of reverse transcriptional polymerase chain reaction (PCR) indicated that the dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP1) genes were significantly tested. Additionally, dentin sialoprotein (DSP) and DMP1 demonstrated significant levels of staining in an immunofluorescence analysis. In contrast, the control cells failed to display the characteristics of odontoblasts. Taken together, these results suggest that hUCMSCs can be induced to differentiate into odontoblast-like cells with TGC-CM and provide a novel strategy for tooth regeneration research.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052618343

Download citation: RISBibTeXText

PMID: 23762828

DOI: 10.1155/2013/218543


Related references

Conditioned media from human adipose tissue-derived mesenchymal stem cells and umbilical cord-derived mesenchymal stem cells efficiently induced the apoptosis and differentiation in human glioma cell lines in vitro. Biomed Research International 2014: 109389, 2014

Conditioned medium from human amniotic epithelial cells may induce the differentiation of human umbilical cord blood mesenchymal stem cells into dopaminergic neuron-like cells. Journal of Neuroscience Research 91(7): 978-986, 2013

Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into germ-like cells in vitro. Journal of Cellular Biochemistry 109(4): 747-754, 2010

Human umbilical cord mesenchymal stem cells may differentiate into Leydig cells through conditioned medium induction. Zhonghua Nan Ke Xue 21(1): 11-16, 2015

Differentiation of human umbilical cord matrix-derived mesenchymal stem cells into germ-like cells. Avicenna Journal of Medical Biotechnology 6(4): 218-227, 2014

Differentiation of human umbilical cord mesenchymal stem cells into germ-like cells in mouse seminiferous tubules. Molecular Medicine Reports 12(1): 819-828, 2015

Sertoli cell-mediated differentiation of male germ cell-like cells from human umbilical cord Wharton's jelly-derived mesenchymal stem cells in an in vitro co-culture system. European Journal of Medical Research 20: 9, 2015

In Vitro Differentiation of Human Umbilical Cord Blood CD133(+)Cells into Insulin Producing Cells in Co-Culture with Rat Pancreatic Mesenchymal Stem Cells. Cell Journal 17(2): 211-220, 2015

Comparison of Human Amniotic, Chorionic, and Umbilical Cord Multipotent Mesenchymal Stem Cells Regarding Their Capacity for Differentiation Toward Female Germ Cells. Cellular Reprogramming 19(1): 44-53, 2017

Experimental study of differentiation of umbilical cord mesenchymal stem cells into smooth muscle cells induced by bladder smooth muscle cells conditioned medium. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 27(12): 1506-1511, 2013

The differentiation potential of gingival mesenchymal stem cells induced by apical tooth germ cell‑conditioned medium. Molecular Medicine Reports 14(4): 3565-3572, 2016

Conditioned umbilical cord tissue provides a natural three-dimensional storage compartment as in vitro stem cell niche for human mesenchymal stroma/stem cells. Stem Cell Research and Therapy 7: 28, 2016

Differentiation of human umbilical cord mesenchymal stem cells into steroidogenic cells in vitro. Experimental and Therapeutic Medicine 12(6): 3527-3534, 2016

Condition medium of HepG-2 cells induces the transdifferentiation of human umbilical cord mesenchymal stem cells into cancerous mesenchymal stem cells. American Journal of Translational Research 8(8): 3429-3438, 2016

Differentiation of Schwann‑like cells from human umbilical cord blood mesenchymal stem cells in vitro. Molecular Medicine Reports 11(2): 1146-1152, 2015