+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Dissection of the role of paeoniflorin in the traditional Chinese medicinal formula Si-Ni-San against contact dermatitis in mice



Dissection of the role of paeoniflorin in the traditional Chinese medicinal formula Si-Ni-San against contact dermatitis in mice



Life Sciences 84(11-12): 337-344



The roles of specific active ingredients in Chinese medicinal formulas have not been clearly elucidated. In this study, we selectively deleted and replenished paeoniflorin from Si-Ni-San, a traditional Chinese prescription, and aimed to identify the molecular basis of how paeoniflorin exerted its effect in Si-Ni-San. Contact dermatitis was induced in mice with picryl chloride. Paeoniflorin was selectively deleted from Si-Ni-San by an immunoaffinity column. Quantitative real-time PCR, western blot, and enzyme-linked immunosorbent assay were used in this study. Both Si-Ni-San and paeoniflorin significantly reduced ear swelling in mice while the paeoniflorin-deleted Si-Ni-San (Si-Ni-San(PF-)) showed little ameliorative effect. In lipopolysaccharide-evoked macrophages, Si-Ni-San and paeoniflorin markedly inhibited tumor necrosis factor-alpha production, cyclooxygenase-2 activity, as well as extracellular signal-regulated kinase 1/2 phosphorylation while Si-Ni-San(PF-) exhibited no or slight inhibitory effect. Furthermore, the inhibitory effect on the production of tumor necrosis factor-alpha reappeared when different proportions of paeoniflorin were replenished in Si-Ni-San(PF-). In addition, the expression of macrophage migration inhibitory factor in T cells, rather than macrophages, was significantly inhibited by Si-Ni-San, but not Si-Ni-San(PF-). Our data indicate paeoniflorin is the principal component of Si-Ni-San, exerting negative regulation on the function of macrophages in contact dermatitis. The present study suggests that dissecting the role of specific constituents in medicinal formulas through selective deletion and replenishment may be a useful strategy in recognizing and validating an active ingredient in traditional Chinese medicine.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 052658001

Download citation: RISBibTeXText

PMID: 19211027

DOI: 10.1016/j.lfs.2008.12.023


Related references

A clinical and patch test study of contact dermatitis from traditional Chinese medicinal materials. Contact Dermatitis. 33(6): 392-392, 1995

Clinical study and patch testing of contact dermatitis caused by traditional Chinese medicinal materials. Zhonghua Pifuke Zazhi 28(5): 293-295, 1995

Screening of the antidepressant-like effect of the traditional Chinese medicinal formula Si-Ni-San and their possible mechanism of action in mice. Pharmacognosy Research 5(1): 36-42, 2013

Inhibition of Neoplastic Transformation and Chemically-Induced Skin Hyperplasia in Mice by Traditional Chinese Medicinal Formula Si-Wu-Tang. Nutrients 9(3):, 2017

Protective effects of FBD--an experimental Chinese traditional medicinal formula on memory dysfunction in mice induced by cerebral ischemia-reperfusion. Journal of Ethnopharmacology 97(3): 477-483, 2005

Regulatory effect of traditional Chinese medicinal formula Zuo-Gui-Wan on the Th17/Treg paradigm in mice with bone loss induced by estrogen deficiency. Journal of Ethnopharmacology 166: 228-239, 2015

Determination of paeoniflorin and geniposide in traditional Chinese medicinal preparation by high performance liquid chromatography. Journal of Food & Drug Analysis 1(2): 191-198, 1993

Effect of bakumijiogan, an herbal formula in traditional Chinese medicine, on atopic dermatitis-like skin lesions induced by mite antigen in NC/Jic mice. Biological & Pharmaceutical Bulletin 31(11): 2108-2113, 2008

Determination of cinnamic acid and paeoniflorin in traditional Chinese medicinal preparations by high-performance liquid chromatography. Journal of Chromatography 593(1-2): 191-199, 1992

SPE-HPLC method for the determination and pharmacokinetic studies on paeoniflorin in rat serum after oral administration of traditional Chinese medicinal preparation Guan-Xin-Er-Hao decoction. Journal of Pharmaceutical and Biomedical Analysis 33(3): 521-527, 2003

Determination of paeoniflorin, ferulic acid and baicalin in the traditional Chinese medicinal preparation Dang-Guei-San by high-performance liquid chromatography. Journal of chromatography A 660(1-2): 299-306, 1994

Determination of cinnamaldehyde, cinnamic acid, paeoniflorin, glycyrrhizin and (6)-gingerol in the traditional Chinese medicinal preparation Kuei-chih-tang by cyclodextrin-modified micellar electrokinetic chromatography. Journal of Chromatography A 771(1-2): 267-274, 1997

Behavioral and biochemical effects of Kaixin-San, a traditional Chinese medicinal empirical formula. Drug Development Research: 5, 267-271, 2008

Role of four major components in the effect of Si-Ni-San, a traditional Chinese prescription, against contact sensitivity in mice. Journal of Pharmacy and Pharmacology 58(9): 1257-1264, 2006

Identification of chemical constituents in traditional Chinese medicine formula using HPLC coupled with linear ion trap-Orbitrap MS from high doses of medicinal materials to equivalent doses of formula: Study on Xiang-Sha-Liu-Jun-Zi-Jia-Jian granules. Journal of Separation Science 39(9): 1619-1627, 2016